[Protective effects of ulinastatin on ischemia/reperfusion injury in the rabbit lung in traumatic hemorrhagic shock].

Abstract

OBJECTIVE:

To investigate the effects and mechanisms of ulinastatin on activities of myeloperoxidase (MPO) in lung tissue and neutrophil elastase (NE) in bronchoalveolar lavage fluid and to evaluate the protective effects of ulinastatin on rabbit lung in traumatic hemorrhagic shock.

METHODS:

Thirty rabbits were randomly assigned to three groups: control group, traumatic hemorrhagic shock group and ulinastatin-treatment group. The traumatic hemorrhagic shock model was reproduced by producing: femur fracture and femoral artery bleeding to reduce the mean artery pressure to (40+/-5) mmHg (1 mmHg=0.133 kPa). The hypotension was maintained 90 minutes before the shed blood and equivalent amount of Ringer's lactate was infused. Four hours after blood volume compensation, the activities of MPO in lung tissue and NE in bronchoalveolar lavage fluid (BALF) were measured, and the extravascular lung water volume was determined.

RESULTS:

Compared with control group, the activities of either MPO in lung tissue or NE in BALF appeared to be increased in the ulinastatin-treatment group (both P<0.05), but their levels were significantly higher in traumatic hemorrhagic shock group(both P<0.05). The extravascular lung water volume was increased significantly in the two experimental group (both P<0.05), however it was more pronounced in traumatic hemorrhagic shock group (all P<0.05).

CONCLUSION:

Ulinastatin can inhibit the increase in the activities of MPO in lung tissue and NE in BALF, and possesses potential protective effects on the lung tissue in traumatic hemorrhagic shock.

• Authors: Gang Wang, Ting-ting Chen, Chang-qing Gao
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 17 Issue 1 Pg. 36-8 (Jan 2005) ISSN: 1003-0603 [Print] China
• PMID: 15636710 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycoproteins
• Peroxidase
• Leukocyte Elastase
• urinastatin

Topics

• Animals
• Disease Models, Animal
• Glycoproteins (pharmacology)
• Leukocyte Elastase (metabolism)
• Lung (blood supply, drug effects, metabolism, pathology)
• Peroxidase (metabolism)
• Rabbits
• Random Allocation
• Reperfusion Injury (metabolism, pathology, prevention & control)
• Shock, Hemorrhagic (metabolism, pathology)
• Shock, Traumatic (metabolism, pathology)