Abstract | OBJECTIVE: To screen genes differentially expressed in two human giant-cell lung cancer lines of same origin but with different metastasis potentials. METHODS: Suppression subtractive hybridization (SSH) was done twice on two giant-cell lung cancer lines, PLA-801C and PLA-801D (hereafter abbreviated as C and D), of same origin but with low (C) and high (D) metastatic potentials. In the first round, SSH C was used as tester and D as driver, while in the second round, the tester and driver were interchanged. The sequences acquired from both rounds of SSH were spotted on glass slides respectively and screened by hybridizing with two-color fluorescence probes. Clones that had different expression levels on chips were also confirmed by RNA dot blot or Northern blot. RESULTS: There were 16 sequences with high expression in C as compared to those in D, and 79 sequences with high expression in D compared to those in C. After sequencing, most of them were found to be highly homologous to those encoding the following proteins: (1) cytokines and their receptors; (2) kinases and related proteins; (3) other proteins including enzymes, heat shock proteins, receptors, proteins of cell skeleton and mitochondria, products of oncogenes, etc; (4) some proteins deduced from gene sequences with yet unknown functions. CONCLUSION:
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Authors | Jin-qiang Zhang, Yan Wang, Tao Wang, Zhi-yan Du, Yuan-ji Xu, Ying-lin Lu |
Journal | Zhonghua zhong liu za zhi [Chinese journal of oncology]
(Zhonghua Zhong Liu Za Zhi)
Vol. 26
Issue 10
Pg. 590-3
(Oct 2004)
ISSN: 0253-3766 [Print] China |
PMID | 15634517
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 14-3-3 Proteins
- HSP70 Heat-Shock Proteins
- Oncogene Proteins
- Proto-Oncogene Proteins
- Receptor Protein-Tyrosine Kinases
- Axl Receptor Tyrosine Kinase
- AXL protein, human
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Topics |
- 14-3-3 Proteins
(metabolism)
- Carcinoma, Giant Cell
(genetics, metabolism, secondary)
- Cell Line, Tumor
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- HSP70 Heat-Shock Proteins
(metabolism)
- Humans
- Lung Neoplasms
(genetics, metabolism, pathology)
- Neoplasm Metastasis
- Nucleic Acid Hybridization
- Oncogene Proteins
(metabolism)
- Proto-Oncogene Proteins
- Receptor Protein-Tyrosine Kinases
(metabolism)
- Axl Receptor Tyrosine Kinase
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