Critical role of the liver CD8+ CD122+ T cells in the generalized Shwartzman reaction of mice.

We examined the role of mouse CD8+ CD122+ T cells, which increase in number with age, in the generalized Shwartzman reaction. This reaction was induced by IL-12 priming and subsequent LPS challenge (after 24 h) in mice of various ages (4-50 weeks of age). Although most young mice (4 or 6 weeks of age) survived, mortality essentially increased with increasing age of the mice, and all mice of 20 weeks of age or older died within 48 h. Serum TNF-alpha levels after LPS challenge also increased age dependently. The neutralization of either IL-12-induced IFN-gamma or LPS-induced TNF-alpha improved the survival of middle-aged (25-week-old) mice. Both IFN-gamma production after IL-12 priming and TNF-alpha production from the liver mononuclear cells after LPS challenge were also prominent in the middle-aged mice. CD8+CD122+ T cells cultured with IL-12 produced a much larger amount of IFN-gamma than CD8+CD122- T cells. Although the depletion of NK/NK T cells did not decrease the IFN-gamma or TNF-alpha production in the Shwartzman reaction of the middle-aged mice, an additional depletion of CD8+CD122+ T cells did decrease such production and also improved mouse survival. Furthermore, young mice transferred with CD8+CD122+ T cells from aged B6 nude mice showed an enhanced Shwartzman reaction.
AuthorsKengo Sato, Manabu Kinoshita, Akira Motegi, Yoshiko Habu, Eiji Takayama, Shigeaki Nonoyama, Hoshio Hiraide, Shuhji Seki
JournalEuropean journal of immunology (Eur J Immunol) Vol. 35 Issue 2 Pg. 593-602 (Feb 2005) ISSN: 0014-2980 [Print] Germany
PMID15627978 (Publication Type: Journal Article)
Chemical References
  • Lipopolysaccharides
  • Receptors, Interleukin-2
  • Tumor Necrosis Factor-alpha
  • Interleukin-12
  • Interferon-gamma
  • Age Factors
  • Animals
  • CD8-Positive T-Lymphocytes (immunology)
  • Interferon-gamma (antagonists & inhibitors, metabolism)
  • Interleukin-12 (metabolism)
  • Kidney (pathology)
  • Kupffer Cells (immunology, metabolism)
  • Lipopolysaccharides (immunology, metabolism)
  • Liver (immunology, pathology)
  • Lung (pathology)
  • Mice
  • Receptors, Interleukin-2 (immunology)
  • Shwartzman Phenomenon (immunology)
  • Time Factors
  • Tumor Necrosis Factor-alpha (antagonists & inhibitors, metabolism)

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