The presence of ascorbate induces expression of brain derived neurotrophic factor in SH-SY5Y neuroblastoma cells after peroxide insult, which is associated with increased survival.

Oxidative stress and free radical production have been implicated in Alzheimer's disease, where low levels of the antioxidant vitamin C (ascorbate) have been shown to be associated with the disease. In this study, neuroblastoma SH-SY5Y cells were treated with hydrogen peroxide in the presence of ascorbate in order to elucidate the mechanism(s) of protection against oxidative stress afforded by ascorbate. Protein oxidation, glutathione levels, cell viability and the effects on the proteome and its oxidized counterpart were monitored. SH-SY5Y cells treated with ascorbate prior to co-incubation with peroxide showed increased viability in comparison to cells treated with peroxide alone. This dual treatment also caused an increase in protein carbonyl content and a decrease in glutathione levels within the cells. Proteins, extracted from SH-SY5Y cells that were treated with either ascorbate or peroxide alone or with ascorbate prior to peroxide, were separated by two-dimensional gel electrophoresis and analyzed for oxidation. Co-incubation for 24 hours decreased the number of oxidised proteins (e.g. acyl CoA oxidase 3) and induced brain derived neurotrophic factor (BDNF) expression. Enhanced expression of BDNF may contribute to the protective effects of ascorbate against oxidative stress in neuronal cells.
AuthorsMelissa M Grant, Vicki S Barber, Helen R Griffiths
JournalProteomics (Proteomics) Vol. 5 Issue 2 Pg. 534-40 (Feb 2005) ISSN: 1615-9853 [Print] Germany
PMID15627972 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antioxidants
  • Brain-Derived Neurotrophic Factor
  • Oxidants
  • Proteins
  • Proteome
  • Hydrogen Peroxide
  • Glutathione
  • Ascorbic Acid
  • Antioxidants (pharmacology)
  • Ascorbic Acid (pharmacology)
  • Brain-Derived Neurotrophic Factor (drug effects, metabolism)
  • Cell Line, Tumor
  • Cell Survival
  • Dose-Response Relationship, Drug
  • Electrophoresis, Gel, Two-Dimensional
  • Enzyme-Linked Immunosorbent Assay
  • Glutathione (analysis, drug effects, metabolism)
  • Humans
  • Hydrogen Peroxide (toxicity)
  • Neuroblastoma (metabolism, pathology)
  • Oxidants (toxicity)
  • Oxidation-Reduction
  • Oxidative Stress (drug effects)
  • Proteins (analysis, chemistry)
  • Proteome (analysis, chemistry)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: