Production of nitric oxide by hypoxic radiosensitizer sanazole.

In this work we investigated the ability of hypoxia-selective radiosensitizer--sanazole to produce nitric oxide (NO).
NO formation was determined by spectophotometric method in the reaction with sanazole and oxyhemoglobin. In suspensions of lymphoma EL-4 and mastocytoma P 8815 cell NO production was estimated indirectly as nitrite concentration in the supernatant fraction.
Transformation of oxyhemoglobin by sanazole to methemoglobin suggested the dissociation of nitro group in aqueous solution and denitration of molecules. Addition of sanazole to hypoxic tumor cell suspension resulted in the increase of nitrite content in tissue culture medium.
Presented data suggest the ability of sanazole to produce NO that may be important in a probable mechanism for antitumor and immunomodulating properties of this radiosensitizer.
AuthorsIrina V Kondakova, Varvara V Tcheredova, Gelena V Zagrebelnaya, Nadezda V Cherdyntseva, Tsutomu V Kagiya, Evgeny L Choinzonov
JournalExperimental oncology (Exp Oncol) Vol. 26 Issue 4 Pg. 329-33 (Dec 2004) ISSN: 1812-9269 [Print] Ukraine
PMID15627070 (Publication Type: Journal Article)
Chemical References
  • Oxyhemoglobins
  • Radiation-Sensitizing Agents
  • Triazoles
  • AK 2123
  • Nitric Oxide
  • Animals
  • Cell Hypoxia
  • Lymphoma (pathology)
  • Mastocytoma (pathology)
  • Mice
  • Mice, Inbred C57BL
  • Neoplasms, Experimental
  • Nitric Oxide (analysis)
  • Oxyhemoglobins (metabolism)
  • Radiation-Sensitizing Agents (pharmacology)
  • Triazoles (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: