Human beta-defensin 3 (hBD-3) expression in A431 cell line and human vulval tumors.

Abstract

UNLABELLED:

The present work was AIMED on the study of human beta-defensin 3 (hBD-3) gene expression in A431 cell line and human vulval tumors.

MATERIALS AND METHODS:

Twenty surgical specimens of both malignant and conventionally normal human vulval tissues have been analyzed for the presence of hBD-3 mRNA by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). In vitro experiments have been carried out on A431 cells.

RESULTS:

It has been shown that hBD-3 gene expression in A431 cells was induced upon EGF stimulation. In human vulval tissues, in 8 cases the expression of hBD-3 gene was registered only in tumor tissue and was absent in paired controls, and 7 cases were characterized by significant increase of hBD-3 gene expression in malignant transformed epithelium in comparison with normal ones. Remaining surgical specimens showed either equal levels of hBD-3 expression or even moderate elevation of hBD-3 expression in normal tissue. In 2 specimens of tumor tissue hBD-3 mRNA was not observed.

CONCLUSION:

In human vulval epithelium, the increase in hBD-3 mRNA expression was predominantly associated with malignant phenotype.

• Authors: Vladimir M Shnitsar, Igor L Lisovskiy, Maria A Soldatkina, Serhiy V Nespryadko, Olexander V Turchak, Alla B Vinnitskaya, Peter V Pogrebniy
• Journal: Experimental oncology (Exp Oncol) Vol. 26 Issue 4 Pg. 328-30 (Dec 2004) ISSN: 1812-9269 [Print] Ukraine
• PMID: 15627069 (Publication Type: Journal Article)

Chemical References

• DEFB103A protein, human
• RNA, Messenger
• beta-Defensins

Topics

• Adult
• Cell Transformation, Neoplastic (genetics)
• Female
• Gene Expression Profiling
• Humans
• Middle Aged
• Phenotype
• RNA, Messenger (analysis, biosynthesis)
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Cells, Cultured
• Vulvar Neoplasms (genetics, pathology)
• beta-Defensins (biosynthesis)