Characterization of neuropeptide F and its receptor from the African malaria mosquito, Anopheles gambiae.

Abstract

The genome of Anopheles gambiae contains sequences encoding a neuropeptide F (Ang-NPF) and NPF receptor (Ang-NPFR) related to the neuropeptide Y signaling family. cDNAs for each were cloned and sequenced. Ang-NPFR was stably expressed for radioligand binding analysis. Ang-NPF exhibited high affinity (IC50 approximately 3 nM) membrane binding; NPFs from Aedes aegypti (Aea-NPF) and Drosophila melanogaster (Drm-NPF) were less potent, with the rank order: Ang-NPF>Aea-NPF>Drm-NPF>Drm-NPF8-36. RT-PCR analysis revealed Ang-NPF and Ang-NPFR transcripts in all life stages. Ang-NPF and Ang-NPFR may be strategically positioned for signaling in relation to nutritional status in the African malaria mosquito.

• Authors: Stephen F Garczynski, Joe W Crim, Mark R Brown
• Journal: Peptides (Peptides) Vol. 26 Issue 1 Pg. 99-107 (Jan 2005) ISSN: 0196-9781 [Print] United States
• PMID: 15626509 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Neuropeptides
• Receptors, Neuropeptide
• neuropeptide F

Topics

• Amino Acid Sequence
• Animals
• Anopheles (genetics, metabolism)
• Base Sequence
• Female
• Larva (metabolism)
• Male
• Molecular Sequence Data
• Neuropeptides (genetics, metabolism)
• Pupa (metabolism)
• Receptors, Neuropeptide (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction