Interleukin-5 reduces the expression of uteroglobin-related protein (UGRP) 1 gene in allergic airway inflammation.

Abstract

Airway inflammation is thought to play a major role in the pathogenesis of bronchial asthma. The precise role of individual inflammatory cells, mediator and asthma related genes in allergic lung diseases is not completely understood. The uteroglobin-related protein (UGRP) 1 was proposed to be an asthma candidate gene and play a role in regulating lung inflammation, however its precise function in the airways remains obscure. In this investigation, we used a mouse model of allergic airway inflammation to establish a relationship between UGRP 1 and IL-5 in airway inflammation. Ovalbumin (OVA) challenged mice demonstrate eosinophilia in airway tissues and high levels of IL-5 in bronchoalveolar lavage (BAL) fluid analogous to that found in bronchial asthma. Interestingly, these "OVA-challenged" mice show down-regulation of Ugrp1 expression as compared with the control group. Regression analysis further demonstrates a significant negative correlation between Ugrp1 mRNA expression in the lung and IL-5 levels in BAL fluid with r = 0.948 and P < 0.0001 when IL-5 levels were normalized by log transformation. Intranasal instillation of IL-5 to mice revealed an inhibitory effect of IL-5 on the expression of Ugrp1 mRNA. Together, these results indicate an involvement of IL-5 in the down-regulation of Ugrp1 expression in airway inflammation such as allergic asthma disease.

• Authors: Yoshihiko Chiba, Achara Srisodsai, Porntip Supavilai, Shioko Kimura
• Journal: Immunology letters (Immunol Lett) Vol. 97 Issue 1 Pg. 123-9 (Feb 15 2005) ISSN: 0165-2478 [Print] Netherlands
• PMID: 15626484 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carrier Proteins
• Interleukin-5
• Proteins
• RNA, Messenger
• SCGB3A2 protein, human
• Scgb3a2 protein, mouse
• Secretoglobins
• Uteroglobin

Topics

• Administration, Intranasal
• Animals
• Carrier Proteins (genetics, metabolism)
• Female
• Gene Expression (drug effects)
• Hypersensitivity (metabolism)
• Interleukin-5 (administration & dosage, genetics, metabolism, pharmacology)
• Mice
• Proteins (genetics, metabolism)
• RNA, Messenger
• Respiratory System (metabolism)
• Secretoglobins
• Uteroglobin