[Biotherapeutic efficacy of adoptive transfer of CD3AK cells in combination with cyclophosphamide and kappa-selenocarrageenan in P 388 leukemic mice].

Abstract

OBJECTIVE:

To study the antileukemia efficacy of a combination of adoptive transfer of CD3AK cells, cyclophosphamide (CTX), and kappa-selenocarrageenan (KSC) in P 388 leukemic mice.

METHODS:

CD3AK cells in normal DBA/2 murine splenocytes were induced with anti-CD3 antibody and low dose recombinant interleukin-2 (rIL-2). The P 388 murine leukemia model was induced by i.p. injection of P 388 cells into normal DBA/2 mice. The tumor-bearing mice were administrated with adoptively transferred CD3AK Cells and/or CTX and/or KSC.

RESULTS:

After tumor inoculation, the cellular immune function of P 388-bearing mice was supressed markedly. Adoptive transfer of CD3AK cells with low dose rIL-2 into the P 388 mice significantly enhanced the splenocyte proliferation (SP) induced by Con A, the NK cell activity and the splenocytic IL-2 production and prolonged their survival (45.19%); CTX (200 mg/kg) alone prolonged the survival of P 388-bearing mice (29.90%), but further decreased the immunodeficiency; combination of CTX and CD3AK passive transfer could prevent the reduction of SP, NK activity and IL-2 production in the leukemic mice and prolonged the survival (59.45%), combination of KSC and adoptively transfected CD2AK cells and/or CTX had a much better therapeutic efficacy for P 388 murine leukemia, 12.50%-75.00% of the leukemic mice were cured.

CONCLUSION:

KSC is a hopeful biological response modifier in cancer biotherapy, and tumor killing effector cells and chemotherapy plus BRM might be a promising candidate for human leukemia biotherapy.

• Authors: H Wei, L Ma
• Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (Zhonghua Xue Ye Xue Za Zhi) Vol. 18 Issue 5 Pg. 243-6 (May 1997) ISSN: 0253-2727 [Print] China
• PMID: 15622755 (Publication Type: Journal Article)

Chemical References

• CD3 Complex
• Interleukin-2
• Organoselenium Compounds
• kappa-selenocarrageenan
• Cyclophosphamide
• Carrageenan

Topics

• Adoptive Transfer
• Animals
• CD3 Complex (metabolism)
• Carrageenan (therapeutic use)
• Cell Line, Tumor
• Cells, Cultured
• Cyclophosphamide (therapeutic use)
• Humans
• Interleukin-2 (biosynthesis)
• Killer Cells, Natural (immunology)
• Leukemia P388 (drug therapy, immunology, metabolism, therapy)
• Male
• Mice
• Organoselenium Compounds (therapeutic use)
• Survival Analysis
• Treatment Outcome