Abstract | OBJECTIVES: Evaluation of HbA1c determination with an automated ion-exchange high-performance liquid chromatography (HPLC) method in patients with clinically silent hemoglobin (Hb) variants. DESIGN AND METHODS: RESULTS: All of these hemoglobin variants caused additional peaks in the chromatograms, without HbA1c results in patients with Hb Graz and Hb Sherwood Forest, and demonstrated extra peaks with HbA1c results that were clinically too low for patients with Hb O Padova and in the patient with HbD. CONCLUSIONS: The development of this automated HPLC method modification with high-resolution beta-thalassemia screening mode aids identification of interference due to some clinically silent Hb variants in HbA(1c) determination.
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Authors | Wolfgang J Schnedl, Theresa Lahousen, Sandra J Wallner, Robert Krause, Rainer W Lipp |
Journal | Clinical biochemistry
(Clin Biochem)
Vol. 38
Issue 1
Pg. 88-91
(Jan 2005)
ISSN: 0009-9120 [Print] United States |
PMID | 15607323
(Publication Type: Journal Article)
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Chemical References |
- Glycated Hemoglobin A
- Hemoglobins, Abnormal
- hemoglobin Graz
- hemoglobin D
- hemoglobin Sherwood Forest
- hemoglobin O Padova
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Topics |
- Chromatography, High Pressure Liquid
- Chromatography, Ion Exchange
- Diabetes Mellitus, Type 1
(blood)
- Diabetes Mellitus, Type 2
(blood)
- Genetic Variation
- Glycated Hemoglobin
(analysis, genetics)
- Hemoglobins, Abnormal
(analysis, genetics)
- Humans
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