Schizophyllan-folate conjugate as a new non-cytotoxic and cancer-targeted antisense carrier.

Abstract

Schizophyllan having folate-appendages was synthesized from native schizophyllan through NaIO(4)-oxidation and the subsequent reductive amination in aqueous ammonia followed by amido-coupling with folic acid. The resulting folate-appended schizophyllan can form stable complex with poly(dA), show specific affinity toward folate binding protein, and mediate effective antisense activity in cancer cells.

• Authors: Teruaki Hasegawa, Tomohisa Fujisawa, Shuichi Haraguchi, Munenori Numata, Ryouji Karinaga, Taro Kimura, Shiro Okumura, Kazuo Sakurai, Seiji Shinkai
• Journal: Bioorganic & medicinal chemistry letters (Bioorg Med Chem Lett) Vol. 15 Issue 2 Pg. 327-30 (Jan 17 2005) ISSN: 0960-894X [Print] England
• PMID: 15603948 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carrier Proteins
• Folate Receptors, GPI-Anchored
• Oligonucleotides, Antisense
• Receptors, Cell Surface
• Sizofiran
• Folic Acid

Topics

• Base Sequence
• Carrier Proteins (chemical synthesis, pharmacology)
• Folate Receptors, GPI-Anchored
• Folic Acid (chemistry, pharmacology)
• Humans
• KB Cells
• Oligonucleotides, Antisense (pharmacology)
• Receptors, Cell Surface
• Sizofiran (chemistry, pharmacology)
• Time Factors