Abstract | OBJECTIVE: The microflora that develops on minocycline strips, used as an adjunct in non-surgical periodontal therapy was studied. DESIGN AND METHODS:
Minocycline (1.4 mg in polycaprolactone vehicle) and control strips were applied into all residual pockets (PD > or = 5mm, > or =2 pockets/subject) of patients with chronic periodontitis 1 month after a course of non-surgical periodontal therapy. Strips were inserted and retained for 3 days, changed to new strips for 3 more days and then removed. Strips were recovered from 14 (eight test, six control) of the 34 participants at day 0 (strip inserted, left for 30 s, removed), days 3 and 6, for (i) anaerobic culture, (ii) coliforms culture, using MacConkey agar, (iii) yeast culture, using Sabouraud's dextrose agar. RESULTS: The mean anaerobic cfu/strip (x10(5); control/test) were 2/6, 24/2, 11/2 at days 0, 3 and 6, respectively (P > 0.05). The corresponding mean proportion of Gram-negative rods and fusiforms were 27%/21%, 27%/15% and 55%/8%. The proportions of Gram-negative rods on test strips by day 6 were significantly reduced (P < 0.05). A significantly increased prevalence of Streptococcus mitis biovar 1 was found on spent test strips (control versus test; 0% versus 38%, Fisher exact test, P = 0.01). Coliform prevalence at days 0, 3 and 6 on control/test strips were 0/13%, 50%/38% and 50%/13%. Yeasts were occasionally isolated. CONCLUSIONS: The findings indicated that the minocycline strips but not the control strip supported a microbial colonisation compatible with periodontal health by day 6.
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Authors | W Keung Leung, Lijian Jin, Joyce Y Y Yau, Qiang Sun, Esmonde F Corbet |
Journal | Archives of oral biology
(Arch Oral Biol)
Vol. 50
Issue 1
Pg. 39-48
(Jan 2005)
ISSN: 0003-9969 [Print] England |
PMID | 15598416
(Publication Type: Journal Article)
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Chemical References |
- Anti-Bacterial Agents
- Pharmaceutical Vehicles
- Minocycline
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Topics |
- Administration, Oral
- Anti-Bacterial Agents
(administration & dosage)
- Bacteria, Aerobic
(isolation & purification)
- Bacteria, Anaerobic
(isolation & purification)
- Chronic Disease
- Colony Count, Microbial
- Enterobacteriaceae
(isolation & purification)
- Female
- Gram-Negative Facultatively Anaerobic Rods
(isolation & purification)
- Humans
- Male
- Middle Aged
- Minocycline
(administration & dosage)
- Periodontal Pocket
(drug therapy, microbiology)
- Periodontium
(microbiology)
- Pharmaceutical Vehicles
- Streptococcus mitis
(isolation & purification)
- Yeasts
(isolation & purification)
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