Abstract |
Expression of the poliovirus receptor (PVR) on cells is a major host determinant of infection by poliovirus. Previously, the only immune cell type known to express PVR was the blood-derived monocyte, which is susceptible to infection at very low frequency. We demonstrate that professional antigen-presenting cells-macrophages and dendritic cells, generated upon differentiation of monocytes-retain expression of PVR and are highly susceptible to infection by type 1 Mahoney strain of poliovirus. Maximal cell-associated titers of virus are obtained within 6 to 8 h postinfection, and cell death and lysis occurs within 24 h postinfection. Similar kinetics are observed in cells infected with the Sabin 1 vaccine strain. Although protein synthesis and receptor-mediated endocytosis are inhibited upon poliovirus infection of these critical antigen-presenting cells, we demonstrate for the first time that functional presentation of antigen occurs in these infected cells via the HLA class II pathway.
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Authors | Rahnuma Wahid, Martin J Cannon, Marie Chow |
Journal | Journal of virology
(J Virol)
Vol. 79
Issue 1
Pg. 401-9
(Jan 2005)
ISSN: 0022-538X [Print] United States |
PMID | 15596833
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Histocompatibility Antigens Class II
- Poliovirus Vaccine, Oral
- Proteins
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Topics |
- Antigen Presentation
- Apoptosis
- CD8-Positive T-Lymphocytes
(immunology)
- Cells, Cultured
- Dendritic Cells
(virology)
- Endocytosis
- Histocompatibility Antigens Class II
(metabolism)
- Humans
- Macrophages
(virology)
- Monocytes
(virology)
- Poliovirus
(pathogenicity)
- Poliovirus Vaccine, Oral
- Proteins
(metabolism)
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