Anti-angiogenesis effect on glioma of attenuated Salmonella typhimurium vaccine strain with flk-1 gene.

Abstract

To investigate the anti-vasculature effects and the anti-glioma effects of attenuated Salmonella typhimurium vaccine strain expressing VEGFR2 (flk-1) gene, plasmid pcDNA3.1-flk1 was constructed and electro-transfected into live attenuated Salmonella typhimurium strain SL7207. Mouse models of intracranial G1261 glioblastoma were treated with an orally administered attenuated Salmonella typhimurium expressing flk-1 gene. The survival period was recorded and vessel density was observed by immunofluorescence. CTLs activity was measured by MTT assay. Our results showed that attenuated Salmonella typhimurium vaccine strain expressing flk-1 gene could significantly inhibit glioblastoma growth, reduce vessel density, prolong the survival period and improve the survival rate in these mice. The flk-1 specific CTLs activity was increased obviously after the vaccination. Our study showed that attenuated Salmonella typhimurium vaccine strain expressing flk-1 gene could break peripheral immune tolerance a in glioma gainst this self-antigen and kill endothelial cells by the orally administered vaccine and can be used for both prophylactic and therapeutic purposes.

• Authors: Keke Feng, Hongyang Zhao, Jian Chen, Dongxiao Yao, Xiaobing Jiang, Wei Zhou
• Journal: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (J Huazhong Univ Sci Technolog Med Sci) Vol. 24 Issue 4 Pg. 389-91 ( 2004) ISSN: 1672-0733 [Print] China
• PMID: 15587406 (Publication Type: Journal Article)

Chemical References

• Angiogenesis Inhibitors
• Salmonella Vaccines
• Vaccines, Attenuated
• Vascular Endothelial Growth Factor Receptor-2

Topics

• Angiogenesis Inhibitors (pharmacology)
• Animals
• Brain Neoplasms (blood supply, therapy)
• Female
• Glioma (blood supply, therapy)
• Mice
• Mice, Inbred C57BL
• Neovascularization, Pathologic
• Salmonella Vaccines (immunology, pharmacology)
• Salmonella typhimurium (immunology)
• Transformation, Genetic
• Vaccines, Attenuated (genetics, immunology, pharmacology)
• Vascular Endothelial Growth Factor Receptor-2 (genetics, immunology)