We studied the urinary excretion of the
isoprostane 8-iso-prostaglandin F(2alpha) as an index of in vivo
oxidant stress, and the production of
leukotriene (LT) B(4) (LTB(4)) by neutrophils in subjects with
chronic obstructive pulmonary disease (
COPD) and normal subjects. Overnight urinary excretion of the
isoprostane was significantly higher in patients with
COPD than in control subjects, and LTB(4) production by challenge of neutrophils obtained from patients with
COPD was also significantly higher than that observed in control neutrophils. Treatment with a standardized
polyphenol extract caused a significant decrease in
isoprostane excretion, accompanied by a statistically significant increase of Pa(O(2)). Furthermore, changes in FEV(1) significantly correlated with the changes in
isoprostane urinary excretion observed from enrollment to the end of treatment. The results of this study suggest that enhanced oxidative stress in subjects with
COPD is paralleled by the increased ability of neutrophils to synthesize the
chemotactic factor LTB(4), and may ultimately contribute to the infiltration/activation of neutrophils into the airways of subjects with
COPD.
Antioxidant treatment in subjects with
COPD is effective in reducing
oxidant stress as shown by the decrease of urinary
isoprostane, a reduction that correlates with the severity of the disease, as indicated by changes in Pa(O(2)) and FEV(1).