Abstract | OBJECTIVE: METHODS: Mature swine were divided into 3 groups: sham thoracotomy, IR (30 minutes of ischemia followed by reperfusion), and ATL ( ischemia/reperfusion with ATL-146e administration for the first 3 hours of reperfusion). Subgroups were killed at 0, 3, 6, 12, 24, and 48 hours after reperfusion. Function was followed up with Tarlov scores. Spinal cord tissue was evaluated for neuronal viability, microtubule-associated protein-2 immunohistochemistry, and neutrophil sequestration ( myeloperoxidase assay). Spinal cord tissue, cerebrospinal fluid, and serum were evaluated for tumor necrosis factor-alpha by enzyme-linked immunosorbent assay. RESULTS: Function was significantly impaired at 24, 36, and 48 hours in the IR group compared with the sham and ATL groups ( P < .05). Neuronal viability and microtubule-associated protein-2 staining were significantly preserved in the sham and ATL groups compared with the IR group at 24 and 48 hours ( P < .05). Spinal cord myeloperoxidase levels were significantly higher in the IR group than in the sham and ATL groups at 24 and 48 hours. Although negligible in serum and cerebrospinal fluid, tumor necrosis factor-alpha levels in the spinal cord peaked significantly higher in the IR group compared with the sham and ATL groups at 6 and 24 hours ( P < .05). CONCLUSIONS:
|
Authors | T Brett Reece, David O Okonkwo, Peter I Ellman, Patrick S Warren, Robert L Smith, A Stewart Hawkins, Joel Linden, Irving L Kron, Curtis G Tribble, John A Kern |
Journal | The Journal of thoracic and cardiovascular surgery
(J Thorac Cardiovasc Surg)
Vol. 128
Issue 6
Pg. 925-32
(Dec 2004)
ISSN: 0022-5223 [Print] United States |
PMID | 15573078
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Microtubule-Associated Proteins
- Receptors, Adenosine A2
- Tumor Necrosis Factor-alpha
- Peroxidase
|
Topics |
- Animals
- Humans
- Microtubule-Associated Proteins
(metabolism)
- Peroxidase
(metabolism)
- Receptors, Adenosine A2
- Spinal Cord Ischemia
(pathology, physiopathology)
- Swine
- Tumor Necrosis Factor-alpha
(metabolism)
|