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Standardization of a neutralizing anti-vaccinia antibodies titration method: an essential step for titration of vaccinia immunoglobulins and smallpox vaccines evaluation.

Abstract
The possibility of mass population vaccination with smallpox vaccine implies the development of anti-vaccinia immunoglobulins for the treatment of severe side effects following vaccination. We have chosen to develop and validate the "gold standard method" (plaque reduction neutralization assay) to titrate neutralizing anti-vaccinia antibodies in two different French laboratories belonging to the Department of Defense (CRSSA) and to the French Health Products Safety Agency (Afssaps). The results of precision, linearity and accuracy of the method led to consider the method as validated. In parallel, we have prepared and lyophilized a pool of anti-vaccinia plasma samples issued from a unique donor and qualified this preparation versus the first British standard to use it as an in-house standard with a titer of 25 international units (IU). This work will allow to titrate, in IU, sera from vaccinated persons in order (i) to titrate purified anti-vaccinia immunoglobulin preparations for vaccine severe side effect treatments; (ii) to investigate the level of neutralizing antibodies in the general population; and (iii) to investigate clinical trials of new generation smallpox vaccines. In the future, this will allow comparability of studies on either smallpox vaccines or on the serological status of the population.
AuthorsIsabelle Leparc-Goffart, Bertrand Poirier, Daniel Garin, Marie-Hélène Tissier, Florence Fuchs, Jean-Marc Crance
JournalJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology (J Clin Virol) Vol. 32 Issue 1 Pg. 47-52 (Jan 2005) ISSN: 1386-6532 [Print] Netherlands
PMID15572006 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Viral
  • Immunoglobulins
  • Smallpox Vaccine
Topics
  • Animals
  • Antibodies, Viral (analysis, blood)
  • Cercopithecus aethiops
  • Evaluation Studies as Topic
  • Immunoglobulins (analysis)
  • Neutralization Tests (methods)
  • Reference Standards
  • Smallpox Vaccine (analysis)
  • Vaccination
  • Vaccinia (diagnosis, immunology)
  • Vaccinia virus (genetics, immunology)
  • Vero Cells

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