Inhibition of phosphoinositide-specific phospholipase C results in the induction of pathogenesis-related genes in soybean.

Abstract

The inositol 1,4,5-trisphosphate (IP3) content is decreased in soybean cells following infection with Pseudomonas syringae pv. glycinea (Psg). In this investigation, a differential display approach was applied to isolate soybean genes that are transcriptionally up-regulated by the inhibition of phosphoinositide-specific phospholipase C (PI-PLC) activity and to study if the transcription of those genes is altered following Psg infection. Four genes, transcriptionally activated following treatment with the PI-PLC-specific inhibitor U-73122, were cloned. Three of the four genes were induced following infection with Psg. The transcripts of a hydrolase homologue (GmHy) were induced in the incompatible but not compatible soybean-Psg interaction. The transcripts of a putative ascorbate oxidase gene (GmAO) were induced in both compatible and incompatible interactions. GmHy and GmAO may represent new classes of pathogenesis-related genes. In addition to these two novel genes, homologues of PR-10 and polygalacturonase inhibitor protein (GmPR10 and GmPGIP, respectively) were identified. These two genes have previously been reported as pathogenesis-related. Transcripts of GmPR-10, but not GmPGIP, were induced in both compatible and incompatible soybean-Psg interactions. Induction of these genes, except for GmPGIP, following inhibition of PI-PLC by either the U-73122 treatment or bacterial infection suggests that PI-PLC may negatively regulate the expression of defence genes.

• Authors: W-M Chou, T Shigaki, C Dammann, Y-Q Liu, M K Bhattacharyya
• Journal: Plant biology (Stuttgart, Germany) (Plant Biol (Stuttg)) Vol. 6 Issue 6 Pg. 664-72 (Nov 2004) ISSN: 1435-8603 [Print] England
• PMID: 15570470 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Plant Proteins
• pathogenesis-related proteins, plant
• Phosphoinositide Phospholipase C
• Phosphatidylinositol Diacylglycerol-Lyase

Topics

• Amino Acid Sequence
• Cloning, Molecular
• Gene Expression Regulation, Plant
• Molecular Sequence Data
• Phosphatidylinositol Diacylglycerol-Lyase (antagonists & inhibitors)
• Phosphoinositide Phospholipase C
• Plant Proteins (genetics, metabolism)
• Soybeans (genetics)
• Transcription, Genetic