Abstract | OBJECTIVES: Cells of the knee meniscus respond to changes in their biochemical and biomechanical environments with alterations in the biosynthesis of matrix constituents and inflammatory mediators. Tumor necrosis factor alpha ( TNF-alpha) is a pro-inflammatory cytokine that is involved in the pathogenesis of both osteoarthritis and rheumatoid arthritis, but its influence on meniscal physiology or mechanobiology is not fully understood. The objectives of this study were to examine the hypothesis that cyclic mechanical strain of meniscal cells modulates the biosynthesis of matrix macromolecules and pro-inflammatory mediators, and to determine if this response is altered by TNF-alpha. METHODS: Cells were isolated from the inner two-thirds of porcine medial menisci and subjected to biaxial tensile strain of 5-15% at a frequency of 0.5Hz. The synthesis of proteoglycan, protein, nitric oxide (NO), and prostaglandin E(2) were determined. RESULTS: CONCLUSIONS: These findings indicate that pro-inflammatory cytokines can modulate the responses of meniscal cells to mechanical signals, suggesting that both biomechanical and inflammatory factors could contribute to the progression of joint disease as a consequence of altered loading of the meniscus.
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Authors | Beverley Fermor, Devon Jeffcoat, Alfred Hennerbichler, David S Pisetsky, J Brice Weinberg, Farshid Guilak |
Journal | Osteoarthritis and cartilage
(Osteoarthritis Cartilage)
Vol. 12
Issue 12
Pg. 956-62
(Dec 2004)
ISSN: 1063-4584 [Print] England |
PMID | 15564062
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Prostaglandins E
- Proteins
- Proteoglycans
- Tumor Necrosis Factor-alpha
- Nitric Oxide Synthase
- Nitric Oxide Synthase Type II
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Topics |
- Animals
- Cells, Cultured
- Dose-Response Relationship, Drug
- Female
- Menisci, Tibial
(cytology, drug effects, metabolism)
- Nitric Oxide Synthase
(biosynthesis)
- Nitric Oxide Synthase Type II
- Prostaglandins E
(biosynthesis)
- Proteins
(metabolism)
- Proteoglycans
(biosynthesis)
- Stress, Mechanical
- Swine
- Tumor Necrosis Factor-alpha
(pharmacology)
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