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[Assessment of polymerase chain reaction (PCR) to diagnose brucellosis in a Brucella infected herd].

Abstract
The diagnosis of bovine brucellosis using PCR in blood and milk samples from two dairy herds were compared to in vitro isolation, complement fixation test (CF), competitive ELISA (C-ELISA) in serum, and indirect ELISA (I-ELISA) in milk. Samples were obtained from 99 cows vaccinated with Brucella abortus strain 19, from a naturally infected herd (A), whose cows were also vaccinated with B. abortus strain RB51 as adults, and 100 from brucellosis free herd (B). In herd A, PCR identified 14 B. abortus infected cows: nine infected with wild type, and five with wild type and RB51, B. abortus S 19 was not identified. B. abortus biotype 1 was isolated from one cow. All cows infected with a wild strain of B. abortus were positive in serologic tests. Brucella was not found in herd B using PCR. Serological test showed 100% sensitivity related to PCR. The specificity for CF, C-ELISA and I-ELISA was 100%, 99% and 95% respectively. PCR could be useful to identify Brucella biotypes and to complement serologic tests.
AuthorsO Lavaroni, N Aguirre, V Vanzini, C Lugaresi, S Torioni de Echaide
JournalRevista Argentina de microbiologia (Rev Argent Microbiol) 2004 Jul-Sep Vol. 36 Issue 3 Pg. 101-6 ISSN: 0325-7541 [Print] Argentina
Vernacular TitleEvaluación de la reacción en cadena de la polimerasa para el diagnóstico de la brucelosis en un rebaño lechero infectado con Brucella spp.
PMID15559190 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Bacterial
  • Bacterial Vaccines
  • DNA, Bacterial
Topics
  • Animals
  • Antibodies, Bacterial (blood)
  • Bacterial Typing Techniques
  • Bacterial Vaccines (immunology)
  • Brucella (genetics, isolation & purification)
  • Brucella abortus (immunology)
  • Brucellosis, Bovine (diagnosis, microbiology)
  • Cattle
  • Complement Fixation Tests
  • DNA, Bacterial (genetics, isolation & purification)
  • Electrophoresis, Agar Gel
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Milk (microbiology)
  • Polymerase Chain Reaction (veterinary)
  • Sensitivity and Specificity
  • Vaccination (veterinary)

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