Study of mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile and their application for immunosensor engineering.

Abstract

Langmuir-Blodgett (LB) technique appears to be quite suitable for generating biospecific surfaces and it has potential application for fabricating biosensors. In this work, mixed Langmuir-Blodgett films of immunoglobulin G/amphiphile have been transferred onto hydrophobic silver surface previously modified by 1-octadecanethiol (ODT) SAMs. In order to obtain stable LB films, the influences of different parameters - type of amphiphile, surface pressure and pH - on the properties of mixed IgG/amphiphile monolayer, were investigated. Electrochemical properties of the engineered immunosensor have been measured by impedimetric spectroscopy. The immunosensor obtained exhibits a high sensitivity and a good specificity in a linear dynamic range from 200 to 1000 ng ml(-1).

• Authors: Yanxia Hou, Chaker Tlili, Nicole Jaffrezic-Renault, Aidong Zhang, Claude Martelet, Laurence Ponsonnet, Abdelhamid Errachid, Josep Samitier, Joan Bausells
• Journal: Biosensors & bioelectronics (Biosens Bioelectron) Vol. 20 Issue 6 Pg. 1126-33 (Dec 15 2004) ISSN: 0956-5663 [Print] England
• PMID: 15556358 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens
• Coated Materials, Biocompatible
• Immunoglobulin G
• Membranes, Artificial

Topics

• Animals
• Antigens (analysis, chemistry, immunology)
• Biosensing Techniques (instrumentation, methods)
• Coated Materials, Biocompatible (chemistry)
• Equipment Design
• Equipment Failure Analysis
• Immunoassay (instrumentation, methods)
• Immunoglobulin G (analysis, chemistry, immunology)
• Membranes, Artificial
• Rabbits