Characteristics of prolinase against various iminodipeptides in erythrocyte lysates from a normal human and a patient with prolidase deficiency.

The effect of various amino acids and MnCl2 on prolinase activity in erythrocyte lysates from a healthy individual and a patient with prolidase deficiency was investigated. A concentration of 0.1 mM MnCl2 increased prolinase activity in normal erythrocytes against pro-gly, pro-glu, pro-leu, pro-ser and pro-phe, but inhibited that against pro-ala, pro-val, pro-met and pro-asp. However, prolinase activity against these iminodipeptides was enhanced by pre-incubation with glycine, independent of MnCl2. The same studies on erythrocytes from a prolidase-deficient patient showed almost the same results as the normal control, except that prolinase activity against pro-gly and pro-ser was slightly inhibited by adding 0.1 mM MnCl2. Some amino acids, glutamic acid and glutamine, slightly enhanced prolinase activity against pro-gly in erythrocytes from both the normal control and the prolidase-deficient patient, but N-acetyl-L-glutamic acid, gamma-aminobutyric acid (GABA) and beta-alanine showed no effect. Branched amino acids, L-valine, L-leucine and L-isoleucine strongly inhibited the prolinase activity against pro-gly. However, conversely, their isomers, D-valine, D-leucine and D-isoleucine, enhanced it. The kinetics of prolinase activity in the erythrocytes from both the normal individual and the prolidasedeficient patient were also studied. Their Km values were changed by adding glycine or 0.1 mM MnCl2, but Vmax values were almost the same.
AuthorsWeifang Wang, Gang Liu, Koichi Yamashita, Masanobu Manabe, Hiroyuki Kodama
JournalClinical chemistry and laboratory medicine (Clin Chem Lab Med) Vol. 42 Issue 10 Pg. 1102-8 ( 2004) ISSN: 1434-6621 [Print] Germany
PMID15552267 (Publication Type: Journal Article)
Chemical References
  • Amino Acids
  • Chlorides
  • Dipeptides
  • Enzyme Inhibitors
  • Imines
  • Manganese Compounds
  • Dipeptidases
  • prolyl dipeptidase
  • manganese chloride
  • Amino Acids (pharmacology)
  • Case-Control Studies
  • Chlorides (pharmacology)
  • Dipeptidases (deficiency, drug effects, metabolism)
  • Dipeptides (blood, chemistry)
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors (pharmacology)
  • Erythrocytes (enzymology)
  • Humans
  • Imines (blood)
  • Isomerism
  • Kinetics
  • Manganese Compounds (pharmacology)

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