Role of synaptojanin 2 in glioma cell migration and invasion.

Abstract

The small GTPase Rac1 is thought to play an important role in cell migration and invasion. We have previously identified synaptojanin 2, a phosphoinositide phosphatase, as an effector of Rac1. Here, we show that small interfering RNA-mediated depletion of either Rac1 or synaptojanin 2 inhibits invasion of SNB19 and U87MG glioblastoma cells through Matrigel and rat brain slices. Depletion of Rac1 or synaptojanin 2 also inhibits migration of SNB19 and U87MG cells on glioma-derived extracellular matrix. In addition, we found that depletion of Rac1 or synaptojanin 2 inhibits the formation of lamellipodia and invadopodia, specialized membrane structures that are thought to be involved in extracellular matrix degradation. These results suggest that synaptojanin 2 contributes to the role of Rac1 in cell invasion and migration by regulating the formation of invadopodia and lamellipodia. This study also identifies synaptojanin 2 as a novel potential target for therapeutic intervention in malignant tumors.

• Authors: Ya-Yu Chuang, Nhan L Tran, Nicole Rusk, Mitsutoshi Nakada, Michael E Berens, Marc Symons
• Journal: Cancer research (Cancer Res) Vol. 64 Issue 22 Pg. 8271-5 (Nov 15 2004) ISSN: 0008-5472 [Print] United States
• PMID: 15548694 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA Primers
• Nerve Tissue Proteins
• synaptojanin
• Phosphoric Monoester Hydrolases

Topics

• Animals
• Base Sequence
• Brain Neoplasms (pathology)
• Cell Line, Tumor
• DNA Primers
• Fluorescent Antibody Technique
• Glioma (pathology)
• Neoplasm Invasiveness
• Nerve Tissue Proteins (physiology)
• Phosphoric Monoester Hydrolases (physiology)
• Rats