Abstract |
2-O-methylisohemigossylic acid lactone, a sesquiterpene, was purified from roots of mokumen (Gossampinus malabarica) and identified by Mass, and (1)H- and (13)-NMR. This sesquiterpene displayed strong growth inhibitory effect against human promyelotic leukemia HL-60 cells. Apoptotic morphological change of the nucleus, including chromatin condensation was induced in the HL-60 cells treated with the sesquiterpene. The fragmentation of DNA by the sesquiterpene to oligonucleosomal-sized fragments, a characteristic of apoptosis, was observed to be dose- and time-dependent in the HL-60 cells. Inhibitors of caspases suppressed the DNA fragmentation induced by the sesquiterpene. These findings suggest that growth inhibition by the sesquiterpene of HL-60 cells results from the induction of apoptosis by the sesqui- terpene, and that caspase cascade is involved in the induction of apoptosis by the compound in the HL-60 cells.
|
Authors | Hiroshige Hibasami, Kazumi Saitoh, Hirotaka Katsuzaki, Kunio Imai, Yue Aratanechemuge, Takashi Komiya |
Journal | International journal of molecular medicine
(Int J Mol Med)
Vol. 14
Issue 6
Pg. 1029-33
(Dec 2004)
ISSN: 1107-3756 [Print] Greece |
PMID | 15547669
(Publication Type: Journal Article)
|
Chemical References |
- 2-O-methylisohemigossylic acid lactone
- Antioxidants
- Chromatin
- Enzyme Inhibitors
- Sesquiterpenes
- Caspases
|
Topics |
- Antioxidants
(pharmacology)
- Apoptosis
(drug effects)
- Bombacaceae
(chemistry)
- Caspases
(metabolism)
- Cell Shape
(drug effects)
- Chromatin
(metabolism)
- Enzyme Inhibitors
(pharmacology)
- HL-60 Cells
- Humans
- Leukemia, Promyelocytic, Acute
(metabolism, pathology)
- Lymphocytes
(drug effects)
- Magnetic Resonance Spectroscopy
- Molecular Structure
- Plant Roots
(chemistry)
- Sesquiterpenes
(chemistry, isolation & purification, pharmacology)
|