| Abstract | Triptolide has been reported to be effective in the treatment of auto-immune diseases. This study investigates the cytotoxic function of triptolide on multiple myeloma (MM) cells. We found that triptolide inhibited the proliferation of both RPMI8226 and U266 cells in a dose-dependent manner (10-80 ng/mL). Triptolide induced apoptosis in MM cells through activation of the cystein protease caspase 8, 9 and 3, and subsequent cleavage of the DNA repair enzyme poly (ADP-ribose) polymerase. Apoptosis was confirmed with cell-cycle analysis and annexin V staining. Moreover, triptolide down-regulated nuclear factor (NF)-kappaB activity in MM cell lines. In addition, triptolide also induced chemosensitivity to doxorubicin and suppressed cell proliferation of fresh MM cells. Therefore, triptolide appears to be a potent inducer of apoptosis in myeloma cells, and might have some benefit in the treatment of myeloma patients. |
| Authors | Lou Yinjun, Jin Jie, Wang Yungui
(Affiliation: Department of Hematology, Institute of Hematology, The First Affiliated Hospital of ZheJiang University, Hangzhou, ZheJiang 310003, China.)
|
| Journal | Leukemia research
(Leuk Res)
Vol. 29
Issue 1
Pg. 99-105
(Jan 2005)
ISSN: 0145-2126 England |
| PMID | 15541481
(Publication Type: Journal Article)
|
| Chemical References |
- Antineoplastic Agents, Alkylating
- Diterpenes
- Epoxy Compounds
- NF-kappa B
- Phenanthrenes
- Proteins
- Transcription Factors
- triptolide
- PARP1 protein, human
- Poly(ADP-ribose) Polymerases
- CASP8 protein, human
- CASP9 protein, human
- Caspase 8
- Caspase 9
- Caspases
|
| Topics |
- Antineoplastic Agents, Alkylating
(pharmacology)
- Apoptosis
(drug effects)
- Caspase 8
- Caspase 9
- Caspases
(metabolism)
- Cell Proliferation
(drug effects)
- Diterpenes
(pharmacology)
- Enzyme Activation
- Epoxy Compounds
- Humans
- Multiple Myeloma
(drug therapy, pathology)
- NF-kappa B
(antagonists & inhibitors)
- Phenanthrenes
(pharmacology)
- Poly(ADP-ribose) Polymerases
- Proteins
(metabolism)
- Transcription Factors
(antagonists & inhibitors)
- Tumor Cells, Cultured
|
