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Characterization of a MAPKK-like protein kinase TOPK.

Abstract
A MAPKK-like protein kinase TOPK expresses in a wide range of proliferating cells and tissues such as cancer cells and testis. However, details of this kinase are still uncovered. We investigated the intracellular distribution of TOPK and its association with cdk1/cyclin B and microtubules. In interphase cells, TOPK expresses in cytosol and nucleus without any significant association with microtubule networks. During mitosis, TOPK-Thr-9 was phosphorylated by cdk1/cyclin B and TOPK significantly associates with mitotic spindles. When TOPK expression was suppressed, formation of spindle midzone was thinned and dimmed and cytokinesis was disturbed. We propose that TOPK plays a role in the formation of spindle midzone and in cytokinesis.
AuthorsSuguru Matsumoto, Yasuhito Abe, Taketsugu Fujibuchi, Takashi Takeuchi, Katsumi Kito, Norifumi Ueda, Kazuhiro Shigemoto, Kiyofumi Gyo
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 325 Issue 3 Pg. 997-1004 (Dec 17 2004) ISSN: 0006-291X [Print] United States
PMID15541388 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Cyclin B
  • Protein Serine-Threonine Kinases
  • Mitogen-Activated Protein Kinase Kinases
  • PDZ-binding kinase
Topics
  • Animals
  • COS Cells
  • Cell Cycle
  • Cell Line, Tumor
  • Chlorocebus aethiops
  • Cyclin B (genetics, metabolism)
  • HeLa Cells
  • Humans
  • Microtubules (metabolism)
  • Mitogen-Activated Protein Kinase Kinases
  • Osteosarcoma (enzymology, pathology)
  • Phosphorylation
  • Protein Serine-Threonine Kinases (genetics, metabolism)
  • Species Specificity

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