Detection of epidermodysplasia verruciformis-associated human papillomavirus DNA in nongenital seborrhoeic keratosis.

Abstract

BACKGROUND:

DNA of epidermodysplasia verruciformis (EV)-associated human papillomaviruses (HPVs) has been widely detected in lesions of malignant skin tumours, benign tumours and other proliferative diseases of epithelial origin.

OBJECTIVES:

To investigate the presence of EV-associated HPV DNA in nongenital seborrhoeic keratosis (SK) and to elucidate the prevalence of distinct HPV genotypes.

METHODS:

We investigated HPV DNA in 55 nongenital SK biopsies, which were compared with 48 normal skin biopsies (healthy controls) using a nested polymerase chain reaction (PCR) using consensus primers CP65/CP70 and CP66/CP69. The positive PCR products were retracted and used to prepare recombination clones with T-vector. Distinct clones were analysed with endonucleases, and HPV genotypes were identified by direct sequencing.

RESULTS:

EV-associated HPV DNA was detected in 42 of 55 (76%) nongenital SK biopsies vs. only 13 of 48 (27%) healthy controls (chi2 = 22.087; P < 0.005). The prevalence was higher in patients with more than five lesions than in those with only one lesion (P < 0.05). Ten distinct HPV genotypes were detected in the nongenital SK biopsies: HPV 20, 23, 5, renal transplant recipient (RTR) X7, HPV 17, 37, 17b, RTRX4, RTRX4b and strain SK3. HPV 20 was found in 26 of 42 (62%) positive specimens, followed by HPV 23 (11 of 42, 26%) and HPV 5 (six of 42, 14%). Existence of multiple HPV genotypes was observed in 12 of 42 (29%) positive specimens. In healthy controls, five genotypes of EV-associated HPV (HPV 20, 23, 5, 17 and RTRX4) were detected, with the same predominant genotype of HPV 20 (five of 13, 38%). Several distinct HPV genotypes were found to coexist in four of 13 (31%) positive specimens.

CONCLUSIONS:

This study provides some evidence that EV-associated HPVs might play a part in the pathogenesis of nongenital SK.

• Authors: Y-H Li, G Chen, X-P Dong, H-D Chen
• Journal: The British journal of dermatology (Br J Dermatol) Vol. 151 Issue 5 Pg. 1060-5 (Nov 2004) ISSN: 0007-0963 [Print] England
• PMID: 15541085 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Viral

Topics

• Adolescent
• Adult
• Age Distribution
• Aged
• Aged, 80 and over
• DNA, Viral (analysis)
• Epidermodysplasia Verruciformis (virology)
• Female
• Genotype
• Humans
• Keratosis, Seborrheic (pathology, virology)
• Male
• Middle Aged
• Papillomaviridae (classification, isolation & purification)
• Papillomavirus Infections (complications, virology)
• Polymerase Chain Reaction (methods)