Abstract | BACKGROUND AND OBJECTIVES: DESIGN AND METHODS: Several parameters indicative of oxidant damage were measured at baseline and following administration of the semi-synthetic flavonoid antioxidant, 7-monohydroxyethylrutoside ( monoHER), to beta-thalassemic mice at a dose of either 500 mg/kg i.p. once a day (n=6) or 250 mg/kg i.p. twice a day (n=6) for 21 days. RESULTS: Significant erythrocyte oxidant damage at baseline was indicated by: (i) dehydration, reduced cell K content, and up-regulated K-Cl co-transport; (ii) marked membrane externalization of phosphatidylserine; (iii) reduced plasma and membrane content of vitamin E; and (iv) increased membrane bound IgG. MonoHER treatment increased erythrocyte K content, and markedly improved all cellular indicators of oxidant stress and of lipid membrane peroxidation. While anemia did not improve, monoHER therapy reduced reticulocyte counts, improved survival of a fraction of red cells, and reduced ineffective erythropoiesis with decreased total bilirubin, lactate dehydrogenase and plasma iron. INTERPRETATION AND CONCLUSIONS:
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Authors | Lucia de Franceschi, Franco Turrini, Marek Honczarenko, Kojio Ayi, Alicia Rivera, Mark D Fleming, Terry Law, Franca Mannu, Frans A Kuypers, Aalt Bast, Wim J F van der Vijgh, Carlo Brugnara |
Journal | Haematologica
(Haematologica)
Vol. 89
Issue 11
Pg. 1287-98
(Nov 2004)
ISSN: 1592-8721 [Electronic] Italy |
PMID | 15531450
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Annexins
- Antioxidants
- Chlorides
- Hydroxyethylrutoside
- Immunoglobulin G
- Vitamin E
- Potassium
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Topics |
- Animals
- Annexins
(blood)
- Antioxidants
(metabolism, therapeutic use)
- Chlorides
(blood)
- Disease Models, Animal
- Erythrocyte Membrane
(metabolism)
- Erythrocytes
(drug effects, metabolism)
- Female
- Hydroxyethylrutoside
(analogs & derivatives, blood, therapeutic use)
- Immunoglobulin G
(blood)
- Ion Transport
- Male
- Mice
- Oxidative Stress
(drug effects)
- Potassium
(blood)
- Vitamin E
(blood)
- beta-Thalassemia
(blood, drug therapy, pathology)
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