Immune responses and protective efficacy in ponies immunised with an equine influenza ISCOM vaccine containing an 'American lineage' H3N8 virus.

Abstract

Protective responses generated by vaccination with an immuno-stimulating complex (ISCOM)-based vaccine for equine influenza (EQUIP F), containing a new 'American lineage' H3N8 virus, were studied. Seven ponies in the vaccine group received two intramuscular injections of EQUIP F given 6 weeks apart. Aerosol challenge with an A/eq/Newmarket/1/93 reference strain 4 weeks after booster vaccination resulted in clinical signs of infection and viral shedding in 7 influenza-naive control animals whereas the vaccinated ponies were significantly protected from both clinical signs and virus excretion. Influenza virus-specific IgG responses in serum following immunisation with the ISCOM vaccine were predominantly of the IgGa and IgGb sub-isotypes, a pattern similar to that generated by equine influenza virus infection. However, in contrast to the response following infection, virus-specific antibody responses in nasal washes following immunisation were characterised by the presence of IgG but not IgA.These results demonstrated that an ISCOM-based vaccine containing A/eq/Kentucky/98 provides strong protective immunity against challenge with an 'American lineage' H3N8 reference virus.

• Authors: C F Crouch, J Daly, D Hannant, J Wilkins, M J Francis
• Journal: Vaccine (Vaccine) Vol. 23 Issue 3 Pg. 418-25 (Dec 02 2004) ISSN: 0264-410X [Print] Netherlands
• PMID: 15530689 (Publication Type: Journal Article)

Chemical References

• Antibodies, Viral
• ISCOMs
• Immunoglobulin A
• Immunoglobulin G
• Influenza Vaccines

Topics

• Animals
• Antibodies, Viral (biosynthesis)
• Female
• Hemolysis
• Horse Diseases (immunology, prevention & control, virology)
• Horses
• ISCOMs (immunology)
• Immunization, Secondary (veterinary)
• Immunoglobulin A (metabolism)
• Immunoglobulin G (blood)
• Influenza A Virus, H3N8 Subtype
• Influenza A virus (immunology)
• Influenza Vaccines (immunology)
• Male
• Nasal Mucosa (immunology, virology)
• Orthomyxoviridae Infections (immunology, prevention & control, veterinary, virology)
• Time Factors
• Vaccination (veterinary)