Effects of amino acids and its metabolites on prolidase activity against various iminodipeptides in erythrocytes from normal human and a patient with prolidase deficiency.

Abstract

BACKGROUND:

The characteristics of prolidase in erythrocytes from controls and patient with prolidase deficiency were investigated.

METHODS:

The erythrocytes were isolated from the heparinized blood of normal human and a patient with prolidase deficiency. Effects of various amino acids and their metabolites on prolidase activity against iminodipeptides in presence of 1 mmol/l MnCl(2) were investigated.

RESULTS:

Prolidase activity against glycylproline in erythrocytes from normal human was strongly enhanced by glycine, L-alanine, L-serine with MnCl(2), but the activity was strongly inhibited by L-valine, and L-leucine. However, the stereoisomers, D-leucine and D-valine enhanced the activity. The prolidase activity against methionylproline in erythrocytes from the patient with prolidase deficiency was also enhanced by glycine, L-alanine and L-serine. The activity was inhibited by l-leucine, but D-leucine and L-valine enhanced the activity against various iminodipeptides.

CONCLUSION:

Prolidase activity against glycylproline in normal human erythrocytes and against methionylproline from the prolidase-deficient patient was enhanced strongly by glycine, alanine and serine with MnCl(2). However, this activity was inhibited by L-leucine, but was enhanced by D-leucine.

• Authors: Gang Liu, Kazuko Nakayama, Shiro Awata, Weifang Wang, Koichi Yamashita, Masanobu Manabe, Hiroyuki Kodama
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 350 Issue 1-2 Pg. 211-7 (Dec 2004) ISSN: 0009-8981 [Print] Netherlands
• PMID: 15530480 (Publication Type: Journal Article)

Chemical References

• Amino Acids
• Chlorides
• Dipeptides
• Imino Acids
• Manganese Compounds
• Dipeptidases
• proline dipeptidase
• manganese chloride

Topics

• Amino Acids (pharmacology)
• Chlorides (blood, metabolism)
• Dipeptidases (deficiency, metabolism)
• Dipeptides (metabolism)
• Dose-Response Relationship, Drug
• Erythrocytes (drug effects, enzymology, metabolism)
• Humans
• Imino Acids (metabolism)
• Manganese Compounds (blood, metabolism)
• Stereoisomerism