Abstract | BACKGROUND & OBJECTIVE: METHODS: Bel-7402, and lymphoma cell lines, U937 and Raji, were cultured with fluorescence-labeled Gal-PEI-c-myc ASODN, and c-myc ASODN. The uptaking rates of Gal-PEI-c-myc ASODN, and intracellular mean fluorescence intensities of Bel-7402 and U937 cells were tested by flow cytometry. Morphology of Gal-PEI-c-myc ASODN and c-myc ASODN entering Bel-7402, U937, and Raji cells was observed under phase-contrast fluorescence microscope. Effects of Gal-PEI-c-myc ASODN of various concentrations on proliferation of these cells were detected by trypan blue dye method. RESULTS: After cultured for 10 min to 4 h, the uptaking rate, and intracellular mean fluorescence intensity of Gal-PEI-c-myc ASODN cultured Bel-7402 cells (88.25%-98.66%, and 38.61%-111.9%) were higher than those of c-myc ASODN cultured Bel-7402 cells, and Gal-PEI-c-myc ASODN cultured U937 cells, significant differences were detected by Poisson test (P < 0.01). Observed by phase-contrast fluorescence microscope, Gal-PEI-c-myc ASODN entered Bel-7402 cells effectively, but can't enter U937, and Raji cells effectively at the same concentration. C-myc ASODN can't enter Bel-7402 cells effectively at the same ASODN concentration. After incubation with Gal-PEI-c-myc ASODN (containing 0.25-1.25 micromol/L of c-myc ASODN) for 48 h, proliferation of Bel-7402 cells was significantly inhibited (P < 0.01=, but no significant differences were detected in U937, and Raji cells (P >0.05). CONCLUSION: Gal-PEI-c-myc ASODN has high targeting delivery effect on Bel-7402 cells, which enhances the intercellular concentration of c-myc ASODN effectively, but it has no such effects on U937, and Raji cells.
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Authors | Jian-Wei Jiang, Yuan Zhang |
Journal | Ai zheng = Aizheng = Chinese journal of cancer
(Ai Zheng)
Vol. 23
Issue 11
Pg. 1288-93
(Nov 2004)
China |
PMID | 15522175
(Publication Type: Comparative Study, English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Oligonucleotides, Antisense
- Polyethyleneimine
- Galactose
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Topics |
- Carcinoma, Hepatocellular
(metabolism, pathology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Drug Delivery Systems
(methods)
- Galactose
(metabolism)
- Genes, myc
(genetics)
- Humans
- Liver Neoplasms
(metabolism, pathology)
- Lymphoma
(metabolism, pathology)
- Oligonucleotides, Antisense
(administration & dosage, pharmacology)
- Polyethyleneimine
(metabolism)
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