[Promotive effect of decreased cyclin E threshold on cell proliferation].

Abstract

BACKGROUND & OBJECTIVE:

Many studies showed that high expression of Cyclin E promotes cell proliferation, but contrary data was also reported that cell proliferation didn't decrease with low expression of Cyclin E. In addition, we observed that many tumor cells have strong capability of proliferation with low expression of Cyclins, including Cyclin E. This study was to analyze effect of reduced Cyclin E threshold on proliferation of acute lymphocyte leukemia cell line MOLT-4 to explain the above phenomena.

METHODS:

We have established the model of decreased Cyclin E threshold in MOLT-4 cells by treating cells with low concentration (5 mmol/L) of caffeine for 2, and 4 h. The positive rates of proliferation cell nuclear antigen (PCNA), Ki67, and DNA strand break induction by photolysis (SBIP) were analyzed by flow cytometry.

RESULTS:

MOLT-4 cells presented sharply decrease of Cyclin E threshold, and increase of positive rates of PCNA, Ki67, and SBIP after treated with caffeine, especially at 2-h point.

CONCLUSIONS:

Decrease of Cyclin E threshold was accompanied by increase of cell proliferation. MOLT-4 cells may remain high proliferation capability with low level of Cyclin expression.

• Authors: Ji-Chao Qin, Xiao-Xi Chen, De-Ding Tao, Yong-Dong Feng, Jun-Bo Hu, Jian-Ping Gong
• Journal: Ai zheng = Aizheng = Chinese journal of cancer (Ai Zheng) Vol. 23 Issue 11 Pg. 1244-8 (Nov 2004) China
• PMID: 15522167 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cyclin E
• Ki-67 Antigen
• Proliferating Cell Nuclear Antigen
• Caffeine

Topics

• Caffeine (pharmacology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cyclin E (biosynthesis, genetics)
• Down-Regulation (drug effects)
• G1 Phase
• Gene Expression Regulation, Leukemic (drug effects)
• Humans
• Ki-67 Antigen (metabolism)
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (genetics, metabolism, pathology)
• Proliferating Cell Nuclear Antigen (metabolism)