Prevention of primary nonfunction after canine liver allotransplantation: the effect of gadolinium chloride.

Abstract

PURPOSE:

Effective suppression of Kupffer cell function is believed to contribute to the prevention of preservation/reperfusion injury. In this study, effect of gadolinium, a synthetic Kupffer cell suppressant, on the reperfusion injury was examined using a canine partial liver transplantation model.

METHODS:

About a 70% partial liver segment was harvested and reimplanted in a mongrel recipient dog weighing 20 to 25 kg. Gadolinium chloride (10 mg/kg) was infused via the cephalic vein 24 hours before harvest of the partial liver (gadolinium group, n = 5). Serum aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and morphologic grading of graft were compared with those of a control group (n = 5). Statistical analysis was done with an independent t-test.

RESULTS:

Average total ischemic time was 4 hours and 27 minutes. At 1 hour after reperfusion, there were no significant differences in AST, ALP, or LDH levels, or pathologic scores. At 48 hours after reperfusion, AST (P = .03) and LDH (P = .05) levels were significantly lower in the gadolinium group.

CONCLUSION:

Kupffer cell blockade using gadolinium chloride may be effective to reduce ischemia reperfusion injury, but the effect is not evident at an early stage of reperfusion.

• Authors: K Y Chung, G Y Jeong, K B Choi, S H Sung, Y S Kim
• Journal: Transplantation proceedings (Transplant Proc) Vol. 36 Issue 7 Pg. 1928-30 (Sep 2004) ISSN: 0041-1345 [Print] United States
• PMID: 15518701 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Gadolinium
• L-Lactate Dehydrogenase
• gadolinium chloride

Topics

• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Dogs
• Gadolinium (therapeutic use)
• Kupffer Cells (drug effects, pathology)
• L-Lactate Dehydrogenase (blood)
• Liver Function Tests
• Liver Transplantation (methods, physiology)
• Transplantation, Homologous