Detection of anti-HLA-F antibodies in sera from cancer patients.

Abstract

BACKGROUND:

We previously reported that a Q5 gene product (Q5 antigen) on the surface of tumor cells caused humoral immune reaction in syngeneic tumor-bearing mice. Transcripts of the Q5 gene were observed in various tumor cells. The human counterpart of the Q5 gene has not been clarified yet.

MATERIALS AND METHODS:

Transcripts of human class Ib genes in various human tumor cell lines were analyzed by the RT-PCR method. Whether anti-HLA-F antibodies were present in sera from cancer patients was investigated through Western blotting with recombinant HLA-F as an antigen.

RESULTS:

Transcripts of the HLA-F gene were produced by various tumor cell lines. In addition, anti-HLA-F IgG was present in the sera derived from various types of cancer patients (26 positives / 42 tested), but not in the sera derived from healthy donors (0 / 20).

CONCLUSION:

Anti-HLA-F antibodies should be further investigated as possible diagnostic tumor markers.

• Authors: Katsuo Noguchi, Manami Isogai, Eri Kuwada, Atsutaka Noguchi, Shigenori Goto, Kohji Egawa
• Journal: Anticancer research (Anticancer Res) 2004 Sep-Oct Vol. 24 Issue 5C Pg. 3387-92 ISSN: 0250-7005 [Print] Greece
• PMID: 15515436 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Neoplasm
• HLA Antigens
• HLA-F antigens
• Histocompatibility Antigens Class I
• Immunoglobulin G
• Recombinant Proteins

Topics

• Adult
• Aged
• Amino Acid Sequence
• Antibodies, Neoplasm (blood, immunology)
• Female
• Gene Expression (immunology)
• Genes, MHC Class I (genetics)
• HLA Antigens (immunology)
• HeLa Cells
• Histocompatibility Antigens Class I (immunology)
• Humans
• Immunoglobulin G (blood, immunology)
• Male
• Middle Aged
• Molecular Sequence Data
• Neoplasms (immunology)
• Recombinant Proteins (biosynthesis, genetics, immunology)