Tumor cell-specific transcription of a murine histocompatibility class Ib Q5 gene.

Abstract

BACKGROUND:

We previously reported that the Q5 gene product (Q5 antigen) was expressed on the surface of various tumor cells derived from H-2k (Qa-2-) mice. The Q5 antigen has tumor-protective antigenicity in the syngeneic mice.

MATERIALS AND METHODS:

Transcripts of the Qa region genes were analyzed by the RT-PCR method. Cell fractionation was performed with the MACS method and the phenotypes were estimated by flow cytometry analysis.

RESULTS:

Transcripts of Q5 were produced by all tumor cell lines. The Q5 transcription was detected only in thymocytes and PBMCs of H-2k (AKR and C3H/He) mice. The phenotype of the PBMCs in which Q5 transcription takes place seems to be, at least partly, CD3-48 cells that might be related to CD3-4-8- spontaneous thymoma cells derived from an H-2k mouse.

CONCLUSION:

The expression of Q5 in tumor cells in general is a result of a change of transcriptional regulation associated with malignant transformation.

• Authors: Katsuo Noguchi, Eri Kuwada, Shigenori Goto, Kohji Egawa
• Journal: Anticancer research (Anticancer Res) 2004 Sep-Oct Vol. 24 Issue 5C Pg. 3379-86 ISSN: 0250-7005 [Print] Greece
• PMID: 15515435 (Publication Type: Journal Article)

Chemical References

• Antigens, Neoplasm
• Antigens, Surface
• H-2 Antigens
• H-2A antigen
• Histocompatibility Antigens Class I
• Q5 antigen, mouse
• RNA

Topics

• Animals
• Antigens, Neoplasm (biosynthesis, genetics)
• Antigens, Surface (biosynthesis, genetics)
• Cell Line, Tumor
• Genes, MHC Class I (genetics)
• H-2 Antigens (biosynthesis)
• Histocompatibility Antigens Class I (biosynthesis, genetics)
• Male
• Mice
• Mice, Inbred AKR
• Mice, Inbred BALB C
• Mice, Inbred C3H
• Mice, Inbred C57BL
• Neoplasms, Experimental (genetics, immunology, metabolism)
• RNA (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Transcription, Genetic