Molecular cloning, sequence analysis and expression of a cDNA encoding human type-1 angiotensin II receptor.

Abstract

We isolated a cDNA encoding type-1 angiotensin II receptor from a human liver cDNA library. The cDNA had an open reading frame encoding a protein of 359 amino acid residues with a relative Mr of 41,060. The deduced amino acid sequence of the human angiotensin II (Ang II) receptor was 95.3% and 94.2% identical to those of bovine and rat type-1 Ang II receptors, respectively, and had a significant similarity with the G protein-coupled receptor. The rank order of the binding to the receptor expressed in COS-7 cells was Ang II greater than Ang III greater than Ang I. The expression of the Ang II receptor mRNA was detected in human liver, lung, adrenal and adrenocortical adenomas but not in adrenomedullary tumor, pheochromocytoma, by Northern blot analysis.

• Authors: R Takayanagi, K Ohnaka, Y Sakai, R Nakao, T Yanase, M Haji, T Inagami, H Furuta, D F Gou, M Nakamuta
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 183 Issue 2 Pg. 910-6 (Mar 16 1992) ISSN: 0006-291X [Print] United States
• PMID: 1550596 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Angiotensin
• Angiotensin II

Topics

• Amino Acid Sequence
• Angiotensin II (genetics)
• Base Sequence
• Binding, Competitive
• Cloning, Molecular
• Gene Expression Regulation, Neoplastic
• Humans
• Molecular Sequence Data
• Receptors, Angiotensin (genetics)
• Restriction Mapping
• Transfection