| Abstract | We have previously demonstrated that vaccination of mice with plasmid DNA vectors expressing immunodominant mycobacterial genes induced cellular immune responses and significant protection against challenge with Mycobacterium tuberculosis. We demonstrate here, using in vitro-synthesized RNA, that vaccination with DNA or RNA constructs expressing the M. tuberculosis MPT83 antigen are capable of inducing specific humoral and T-cell immune responses and confer modest but significant protection against M. tuberculosis challenge in mice. This is the first report of protective immunity conferred against intracellular bacteria by an RNA vaccine. This novel approach avoids some of the drawbacks of DNA vaccines and illustrates the potential for developing new antimycobacterial immunization strategies. |
| Authors | Tian Xue, Evangelos Stavropoulos, Min Yang, Silvia Ragno, Martin Vordermeier, Mark Chambers, Glyn Hewinson, Douglas B Lowrie, M Joseph Colston, Ricardo E Tascon
(Affiliation: Mycobacterial Division, National Institute for Medical Research, London NW7 1AA, UK.)
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| Journal | Infection and immunity
(Infect Immun)
Vol. 72
Issue 11
Pg. 6324-9
(Nov 2004)
ISSN: 0019-9567 United States |
| PMID | 15501761
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Antibodies, Bacterial
- Antigens, Bacterial
- Bacterial Proteins
- MPB83 protein, Mycobacterium
- Membrane Proteins
- RNA, Bacterial
- RNA, Messenger
- Tuberculosis Vaccines
- Vaccines, DNA
- RNA Replicase
|
| Topics |
- Amino Acid Sequence
- Animals
- Antibodies, Bacterial
(blood)
- Antigens, Bacterial
(genetics, immunology, metabolism)
- Bacterial Proteins
(genetics, immunology, metabolism)
- Cell Line
- Cricetinae
- Female
- Membrane Proteins
(genetics, immunology, metabolism)
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Mycobacterium tuberculosis
(genetics, immunology)
- RNA Replicase
(administration & dosage, genetics, immunology)
- RNA, Bacterial
(genetics, immunology, metabolism)
- RNA, Messenger
(genetics, immunology, metabolism)
- Sindbis Virus
(enzymology, genetics)
- T-Lymphocytes
(immunology)
- Transfection
- Tuberculosis Vaccines
(administration & dosage, genetics, immunology)
- Tuberculosis, Pulmonary
(immunology, microbiology, prevention & control)
- Vaccination
- Vaccines, DNA
(administration & dosage, immunology)
|
