[Study on effect of berbamine on multidrug resistance leukemia K562/Adr cells].

Abstract

OBJECTIVE:

To study the effect and mechanism of berbamine on the apoptosis of multidrug resistant leukemia K562/Adr cells and in reversing the drug resistance.

METHODS:

IC50 value of K562/Adr cell was determined with MTT method, cell apoptosis rate was analyzed by flow cytometry with Annexin V FITC-PI assay, with the peak and cell cycle detected by PI staining. At the same time, flow cytometry was also used in determining Caspase-3, P-GP protein expression and drug accumulating capacity in cells, and RT-PCR method was used to analyze the gene expression of mdr-1.

RESULTS:

Berbamine could inhibit human leukemia K562/Adr cell growth in dose-dependent manner, it could also induce cell apoptosis, increase the protein expression of Caspase-3 and the drug excretion capacity of cells, reduce the mRNA and protein expression levels of mdr-1 gene.

CONCLUSION:

Berbamine could activate Caspase-3 to induce human leukemia K562/Adr cell apoptosis, and by reducing mdr-1 gene expression to reverse its multidrug resistance.

• Authors: Qing-hua Dong, Shu Zheng, Rong-zhen Xu, Qinghua Lu, Liming He
• Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine (Zhongguo Zhong Xi Yi Jie He Za Zhi) Vol. 24 Issue 9 Pg. 820-2 (Sep 2004) ISSN: 1003-5370 [Print] China
• PMID: 15495829 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATP Binding Cassette Transporter, Subfamily B, Member 1
• Alkaloids
• Antineoplastic Agents, Phytogenic
• Benzylisoquinolines
• RNA, Messenger
• CASP3 protein, human
• Caspase 3
• Caspases
• berbamine

Topics

• ATP Binding Cassette Transporter, Subfamily B, Member 1 (biosynthesis, genetics)
• Alkaloids (pharmacology)
• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis (drug effects)
• Benzylisoquinolines (pharmacology)
• Caspase 3
• Caspases (biosynthesis, genetics)
• Drug Resistance, Neoplasm (drug effects, genetics)
• Humans
• K562 Cells
• RNA, Messenger (biosynthesis, genetics)