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Distribution and subcellular localization of a water-soluble hematoporphyrin-platinum(II) complex in human bladder cancer cells.

Abstract
The water-soluble porphyrin-platinum complex diammine[7,12-bis[1-(polyethyleneglycol-750-monomethylether-1-yl)ethyl]-3,8,13,17-tetramethylporphyrin-2,18-dipropionato]platinum(II) (PEG-HPPt) was studied with respect to cellular accumulation, subcellular localization, behavior in 3D-cell aggregates and degree of DNA platination on the low-differentiated J82 cells, a model of invasive bladder cancer, and UROtsa, a normal urothelial cell line. Accumulation studies with 2D and spheroid cell cultures revealed that the concentration of PEG-HPPt was 1.7-times higher in J82 cancer cells than in UROtsa cells. Despite its high molecular weight, penetration of PEG-HPPt was not restricted to the peripheral cells of the spheroids. Fluorescence microscopic analysis showed that PEG-HPPt was localized in essential cellular targets of photodynamic therapy. DNA platination in J82 and UROtsa cells was higher by PEG-HPPt than by cisplatin, whereas there was no significant difference between the two cell lines.
AuthorsChristian Lottner, Ruth Knuechel, Guenther Bernhardt, Henri Brunner
JournalCancer letters (Cancer Lett) Vol. 215 Issue 2 Pg. 167-77 (Nov 25 2004) ISSN: 0304-3835 [Print] Ireland
PMID15488635 (Publication Type: Comparative Study, Evaluation Study, Journal Article)
Chemical References
  • Antineoplastic Agents
  • DNA, Neoplasm
  • Hematoporphyrins
  • Organoplatinum Compounds
  • Photosensitizing Agents
  • Porphyrins
  • diammine(7,12-bis(1-(polyethyleneglycol-750-monomethylether-1-yl)ethyl)-3,8,13,17-tetramethylporphyrin-2,18-dipropionato)platinum(II)
  • Platinum
Topics
  • Antineoplastic Agents (metabolism, therapeutic use)
  • Cell Line
  • Cell Line, Tumor
  • DNA, Neoplasm (metabolism)
  • Drug Screening Assays, Antitumor
  • Hematoporphyrins (metabolism, therapeutic use)
  • Humans
  • Organoplatinum Compounds (chemistry, metabolism, therapeutic use)
  • Photosensitizing Agents (metabolism, therapeutic use)
  • Platinum (metabolism)
  • Porphyrins (chemistry, therapeutic use)
  • Spheroids, Cellular
  • Tissue Distribution
  • Urinary Bladder (metabolism)
  • Urinary Bladder Neoplasms (drug therapy, metabolism)

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