HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Protein translation inhibition by Stachybotrys chartarum conidia with and without the mycotoxin containing polysaccharide matrix.

Abstract
Recent studies have correlated the presence of Stachybotrys chartarum in structures with SBS. S. chartarum produces mycotoxins that are thought to produce some of the symptoms reported in sick-building syndrome (SBS). The conidia (spores) produced by Stachybotrys species are not commonly found in the air of buildings that have been found to contain significant interior growth of this organism. This could be due in part to the large size of the Stachybotrys spores, or the organism growing in hidden areas such as wall cavities. However, individuals in buildings with significant Stachybotrys growth frequently display symptoms that may be attributed to exposure to the organism's mycotoxins. In addition, Stachybotrys colonies produce a "slime" or polysaccharide (carbohydrate) matrix that coats the hyphae and the spores. The intent of this project was to determine whether the carbohydrate matrix and the mycotoxins embedded in it could be removed from the spores by repeated washings with either aqueous or organic solvents. The results demonstrated that the process of spore washing removed compounds that were toxic in a protein translation assay as compared to spores that were washed with an organic solution, however a correlation between carbohydrate removal during the washing process and the removal of mycotoxins from the spore surface was not observed. These data demonstrated that mycotoxins are not likely to be found exclusively in the carbohydrate matrix of the spores. Therefore, mycotoxin removal from the spore surface can occur without significant loss of polysaccharide. We also showed that toxic substances may be removed from the spore surface with an aqueous solution. These results suggest that satratoxins are soluble in aqueous solutions without being bound to water-soluble moieties, such as the carbohydrate slime matrix.
AuthorsEnusha Karunasena, J Danny Cooley, Douglas Straus, David C Straus
JournalMycopathologia (Mycopathologia) Vol. 158 Issue 1 Pg. 87-97 (Jul 2004) ISSN: 0301-486X [Print] Netherlands
PMID15487326 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Polysaccharides, Bacterial
  • Protein Synthesis Inhibitors
  • Trichothecenes
  • satratoxin G
  • satratoxin H
  • Methanol
Topics
  • Animals
  • Chromatography, High Pressure Liquid
  • Humans
  • Methanol (chemistry)
  • Polysaccharides, Bacterial (chemistry, metabolism)
  • Protein Synthesis Inhibitors (isolation & purification, metabolism, pharmacology)
  • Rabbits
  • Reticulocytes (drug effects, metabolism)
  • Sick Building Syndrome (microbiology)
  • Spores, Fungal (metabolism)
  • Stachybotrys (metabolism)
  • Trichothecenes (isolation & purification, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: