Detection of bcl-2 and bax expression and bcl-2/JH fusion gene in intrahepatic cholangiocarcinoma.

Abstract

AIM:

To investigate the relationship between bcl-2 gene and its related protein bax and intrahepatic cholangiocellular carcinoma (CCC).

METHODS:

Semi-nested in situ PCR (SNISPCR) and immunohistochemistry were performed to detect bcl-2/JH fusion gene and bcl-2, bax protein expression in 29 cases of CCC.

RESULTS:

No bcl-2/JH fusion gene was found in all cases of CCC, 72.4% of 29 cases expressed bcl-2 protein. Bcl-2 protein expression was related to histopathological grades (P<0.05). There was no corresponding relationship between bcl-2/JH fusion gene formation and bcl-2 protein expression in CCC (P<0.05). Bax was expressed in 10.3% of 29 cases. The ratio of bcl-2 to bax in normal liver tissues (3.5 to 1) was different from that in tumor tissues (7.0 to 1).

CONCLUSION:

It is suggested that bcl-2/JH fusion gene formation is not a frequent event and may not play an important role in the pathogenesis of CCC. However, aberrant ratio of bcl-2 to bax protein expression may be involved in the course of tumorigenesis of CCC. Abnormal bcl-2 protein expression may not be solely resulted from bcl-2/JH fusion gene.

• Authors: Lin-Lang Guo, Sha Xiao, Ying Guo
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 10 Issue 22 Pg. 3251-4 (Nov 15 2004) ISSN: 1007-9327 [Print] United States
• PMID: 15484294 (Publication Type: Journal Article)

Chemical References

• BAX protein, human
• Immunoglobulin Heavy Chains
• Oncogene Proteins, Fusion
• Proto-Oncogene Proteins c-bcl-2
• bcl-2-Associated X Protein

Topics

• Bile Duct Neoplasms (genetics, physiopathology)
• Bile Ducts, Intrahepatic
• Cholangiocarcinoma (genetics, physiopathology)
• Gene Expression Regulation, Neoplastic
• Humans
• Immunoglobulin Heavy Chains (genetics, metabolism)
• Immunohistochemistry
• Oncogene Proteins, Fusion (genetics, metabolism)
• Polymerase Chain Reaction
• Proto-Oncogene Proteins c-bcl-2 (genetics, metabolism)
• bcl-2-Associated X Protein