The gene expression profile of cyst epithelial cells in autosomal dominant polycystic kidney disease patients.

Abstract

Autosomal dominant polycystic kidney disease (ADPKD) is a common genetic disorder characterized by the formation of fluid-filled cysts in the kidney and progressive renal failure. Other manifestations of ADPKD include the formation of cysts in other organs (liver, pancreas, and spleen), hypertension, cardiac defects, and cerebral aneurysms. The loss of function of the polycystin -1 and -2 results in the formation of epithelium-lined cysts, a process that depends on initial epithelial proliferation. cDNA microarrays powerfully monitor gene expression and have led to the discoveries of pathways regulating complex biological processes. We undertook to profile the gene expression patterns of epithelial cells derived from the cysts of ADPKD patients using the cDNA microarray technique. Candidate genes that were differently expressed in cyst tissues were identified. 19 genes were up-regulated, and 6 down-regulated. Semi-quantitative RT-PCR results were consistent with the microarray findings. To distinguish between normal and epithelial cells, we used the hierarchical method. The results obtained may provide a molecular basis for understanding the biological meaning of cytogenesis.

• Authors: Jae Eun Lee, Min Ha Park, Jong Hoon Park
• Journal: Journal of biochemistry and molecular biology (J Biochem Mol Biol) Vol. 37 Issue 5 Pg. 612-7 (Sep 30 2004) ISSN: 1225-8687 [Print] Korea (South)
• PMID: 15479626 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Membrane Proteins
• TRPP Cation Channels
• polycystic kidney disease 2 protein

Topics

• Cells, Cultured
• Down-Regulation
• Epithelial Cells (metabolism)
• Gene Expression
• Gene Expression Profiling
• Humans
• Membrane Proteins (genetics, metabolism)
• Polycystic Kidney, Autosomal Dominant (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• TRPP Cation Channels
• Up-Regulation