Abstract |
Migration and maturation of human dendritic cells derived from CD34+ progenitor cells (DC) infected by Toxoplasma gondii were studied in an in vitro model. We demonstrated that infection with virulent type I strains RH and ENT or type II low virulent strains PRU and CAL induced DC migration towards MIP-3beta. However, type II strains induced a higher percentage of migrating cells than that induced by type I strains or positive controls (chemical allergen or lipopolysaccharides). Type II strains produced soluble factors responsible of the high migration whereas heat killed tachyzoites did not induced a migration higher than positive controls. We also demonstrated that infection by virulent strains and not by type II stains or heat killed tachyzoites triggers DC maturation. A soluble factor released by type II strains was responsible of the absence of DC maturation. Taken together, these results demonstrated that the interference of T. gondii in the behaviour of DC functions is related to the strain types and can be supported by secretion of soluble factors by the parasite.
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Authors | Julien Diana, Florence Persat, Marie-Jeanne Staquet, Olga Assossou, Josette Ferrandiz, Marie-Jo Gariazzo, François Peyron, Stéphane Picot, Daniel Schmitt, Claude Vincent |
Journal | FEMS immunology and medical microbiology
(FEMS Immunol Med Microbiol)
Vol. 42
Issue 3
Pg. 321-31
(Nov 01 2004)
ISSN: 0928-8244 [Print] England |
PMID | 15477046
(Publication Type: Journal Article)
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Chemical References |
- Antigens, CD
- Antigens, CD34
- B7-2 Antigen
- CCL19 protein, human
- CCR6 protein, human
- CCR7 protein, human
- CD83 antigen
- CD86 protein, human
- Chemokine CCL19
- Chemokines, CC
- HLA-DR Antigens
- Immunoglobulins
- Lysosome-Associated Membrane Glycoproteins
- Membrane Glycoproteins
- Receptors, CCR6
- Receptors, CCR7
- Receptors, Chemokine
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Topics |
- Animals
- Antigens, CD
(analysis)
- Antigens, CD34
(analysis)
- B7-2 Antigen
- Cell Differentiation
- Cell Line
- Cell Movement
- Chemokine CCL19
- Chemokines, CC
(immunology)
- Coculture Techniques
- Dendritic Cells
(immunology, parasitology, physiology)
- HLA-DR Antigens
(analysis)
- Humans
- Immunoglobulins
(analysis)
- Lysosome-Associated Membrane Glycoproteins
- Membrane Glycoproteins
(analysis)
- Monocytes
- Phenotype
- Receptors, CCR6
- Receptors, CCR7
- Receptors, Chemokine
(analysis)
- Toxoplasma
(immunology, metabolism, pathogenicity)
- Virulence
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