Extra copies of chromosome 2 are a recurring aberration in ALK-negative lymphomas with anaplastic morphology.

Abstract

The purpose of this study was to evaluate fluorescence in situ hybridization abnormalities of the 2p23 anaplastic lymphoma kinase (ALK) gene loci in lymphomas with anaplastic morphology. We studied 24 anaplastic large cell lymphomas (ALCL) classified by World Health Organization criteria [17 primary nodal/systemic (10 ALK+, 7 ALK-), seven primary cutaneous], and 17 additional non-Hodgkin's lymphomas [one ALK+ B-lineage lymphoma, 14 ALK- diffuse large B-cell lymphomas (seven anaplastic variants, five nonanaplastic, two secondary CD30+), two follicular lymphomas]. ALK- lymphomas with anaplastic morphology showed extra nonrearranged anaplastic lymphoma kinase gene loci (P=0.004) due to trisomy 2 irrespective of the following factors: B or T/null phenotype (P=0.315), diagnostic categories of systemic or cutaneous ALCL or the above-mentioned B-cell lymphomas (P=0.131), and CD30 positivity by immunohistochemistry (P=1.000). Trisomy 2 was absent in all ALK+ lymphomas (P=0.009), which showed rearranged ALK gene loci (P<0.001). Whether trisomy 2 is a primary or secondary event that leads to ALK- lymphomas cannot be determined from this study. Its presence in secondary B-cell lymphomas suggests that trisomy 2 may be a secondary cytogenetic aberration in lymphomas in general. Further investigation of this finding is necessary to further our understanding of the heterogeneous group of ALK- lymphomas.

• Authors: Rina Kansal, Sheila N J Sait, AnneMarie W Block, Pamela M Ward, Felicity L R Kelly, Richard T Cheney, Myron Czuczman, Martin L Brecher, Maurice Barcos
• Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (Mod Pathol) Vol. 18 Issue 2 Pg. 235-43 (Feb 2005) ISSN: 0893-3952 [Print] United States
• PMID: 15475930 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Ki-1 Antigen
• Lewis X Antigen
• Mucin-1
• ALK protein, human
• Anaplastic Lymphoma Kinase
• Protein-Tyrosine Kinases
• Receptor Protein-Tyrosine Kinases

Topics

• Adult
• Anaplastic Lymphoma Kinase
• Child
• Chromosome Aberrations
• Chromosomes, Human, Pair 2 (genetics)
• Female
• Humans
• Immunohistochemistry
• In Situ Hybridization, Fluorescence
• Ki-1 Antigen (analysis)
• Lewis X Antigen (analysis)
• Lymphoma, B-Cell (genetics, metabolism, pathology)
• Lymphoma, Large B-Cell, Diffuse (genetics, metabolism, pathology)
• Male
• Mucin-1 (analysis)
• Protein-Tyrosine Kinases (analysis, genetics)
• Receptor Protein-Tyrosine Kinases
• Statistics as Topic