Transcriptome analysis in a rat model of L-DOPA-induced dyskinesia.

Abstract	We have examined the pattern of striatal messenger RNA expression of over 8000 genes in a rat model of levodopa (L-DOPA)-induced dyskinesia and Parkinson disease (PD). 6-Hydroxydopamine (6-OHDA)-lesioned rats were treated with L-DOPA or physiological saline for 22 days and repeatedly tested for antiakinetic response to L-DOPA and the development of abnormal involuntary movements (AIMs). In a comparison of rats that developed a dyskinetic motor response to rats that did not, we found striking differences in gene expression patterns. In rats that developed dyskinesia, GABA neurons had an increased transcriptional activity, and genes involved in Ca2+ homeostasis, in Ca2+ -dependent signaling, and in structural and synaptic plasticity were upregulated. The gene expression patterns implied that the dyskinetic striatum had increased transcriptional, as well as synaptic activity, and decreased capacity for energy production. Some basic maintenance chores such as ribosome protein biosynthesis were downregulated, possibly a response to expended ATP levels.
Authors	Christine Konradi, Jenny E Westin, Manolo Carta, Molly E Eaton, Katarzyna Kuter, Andrzej Dekundy, Martin Lundblad, M Angela Cenci
Journal	Neurobiology of disease (Neurobiol Dis) Vol. 17 Issue 2 Pg. 219-36 (Nov 2004) ISSN: 0969-9961 [Print] United States
PMID	15474360 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Carrier Proteins Dopamine Agents Ions Microfilament Proteins Neurotransmitter Agents RNA, Messenger Receptors, Cell Surface Levodopa Phosphotransferases Phosphoric Monoester Hydrolases Calcium-Transporting ATPases
Topics	Animals Calcium-Transporting ATPases (genetics) Carrier Proteins (genetics) Corpus Striatum (metabolism) Cytoskeleton (metabolism) Dopamine Agents Dyskinesia, Drug-Induced (genetics, metabolism) Energy Metabolism (genetics) Gene Expression Gene Expression Profiling Homeostasis Ions Levodopa Microfilament Proteins (genetics) Mitochondria (enzymology) Multigene Family Neurotransmitter Agents (biosynthesis, genetics) Oligonucleotide Array Sequence Analysis Phosphoric Monoester Hydrolases (genetics) Phosphotransferases (genetics) RNA, Messenger (metabolism) Rats Rats, Sprague-Dawley Receptors, Cell Surface (genetics) Synapses

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!