Abstract | BACKGROUND: METHODS: For these experiments, a murine model with artificial wounds was used. Forty mice were randomly divided into two groups according to the substance to be injected into a wound: a sodium hyaluronate group and a control group. Tumors were created by inoculation of transplantable adenocarcinoma cell line colon 26. Two weeks later, the size, weight, proliferating cell nuclear antigen-labeling index, and CD44 expression of the subcutaneous tumors were compared between the two groups of mice. RESULTS: There were significantly greater increases in the growth and the weight of subcutaneous tumors in the sodium hyaluronate group compared with the control group. The PCNA-labeling index of cancer cells also was higher in the sodium hyaluronate group. Immunohistochemistry and Western blot analysis demonstrated that the CD44 protein expression of cancer cells was higher in the sodium hyaluronate group vs. the control group. CONCLUSIONS:
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Authors | Yoko Matsui, Masafumi Inomata, Koichi Izumi, Kazuya Sonoda, Norio Shiraishi, Seigo Kitano |
Journal | Gastrointestinal endoscopy
(Gastrointest Endosc)
Vol. 60
Issue 4
Pg. 539-43
(Oct 2004)
ISSN: 0016-5107 [Print] United States |
PMID | 15472675
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Hyaluronan Receptors
- Proliferating Cell Nuclear Antigen
- Hyaluronic Acid
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Topics |
- Animals
- Blotting, Western
- Cell Division
(drug effects)
- Cell Line, Tumor
- Endoscopy
(methods)
- Female
- Hyaluronan Receptors
(analysis)
- Hyaluronic Acid
(adverse effects, pharmacology)
- Immunohistochemistry
- Mice
- Mice, Inbred BALB C
- Mucous Membrane
(surgery)
- Neoplasms
(pathology)
- Proliferating Cell Nuclear Antigen
(analysis)
- Random Allocation
- Staining and Labeling
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