Helicobacter pylori infection causes persistent platelet activation in vivo through enhanced lipid peroxidation.

Abstract

OBJECTIVE:

We aimed at investigating the relationship between Helicobacter pylori infection and in vivo lipid peroxidation and platelet activation, as reflected by urinary 8-iso-prostaglandin (PG)F(2alpha) and 11-dehydro-thromboxane (TX)B2, respectively, in otherwise healthy dyspeptic subjects.

METHODS AND RESULTS:

We measured urinary 8-iso-PGF2alpha and 11-dehydro-TXB2 excretion in 40 dyspeptic subjects with a positive 13C-urea breath test and 38 dyspeptic individuals with a negative test. Moreover, we investigated the effects of H pylori eradication on prostanoid metabolite excretion in 23 H pylori-positive subjects. We also measured prostanoid metabolite excretion before and after selective cyclooxygenase-2 inhibition with rofecoxib in 4 H pylori-positive subjects. Urinary 8-iso-PGF2alpha and 11-dehydro-TXB2 excretion was significantly higher in the H pylori-positive individuals than in controls. A significant direct correlation was found between the degree of positivity to the 13C-urea breath test and urinary 8-iso-PGF2alpha excretion. The latter was linearly correlated with urinary 11-dehydro-TXB2. Successful eradication of H pylori infection led to a significant reduction in both 8-iso-PGF(2alpha) and 11-dehydro-TXB2. Furthermore, their levels were unaffected after treatment with rofecoxib.

CONCLUSIONS:

Our study provides evidence of enhanced in vivo lipid peroxidation and platelet activation in association with H pylori infection and suggests a novel mechanism by which an infectious agent could contribute to atherothrombosis.

• Authors: Giovanni Davì, Matteo Neri, Angela Falco, Davide Festi, Tea Taraborelli, Giovanni Ciabattoni, Stefania Basili, Franco Cuccurullo, Carlo Patrono
• Journal: Arteriosclerosis, thrombosis, and vascular biology (Arterioscler Thromb Vasc Biol) Vol. 25 Issue 1 Pg. 246-51 (Jan 2005) ISSN: 1524-4636 [Electronic] United States
• PMID: 15472127 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Cyclooxygenase 2 Inhibitors
• Cyclooxygenase Inhibitors
• Membrane Proteins
• 8-epi-prostaglandin F2alpha
• Thromboxane B2
• 11-dehydro-thromboxane B2
• Dinoprost
• Cyclooxygenase 2
• PTGS2 protein, human
• Prostaglandin-Endoperoxide Synthases

Topics

• Adult
• Aged
• Cross-Sectional Studies
• Cyclooxygenase 2
• Cyclooxygenase 2 Inhibitors
• Cyclooxygenase Inhibitors (pharmacology)
• Dinoprost (analogs & derivatives, metabolism, urine)
• Fasting (physiology, urine)
• Helicobacter Infections (drug therapy, pathology, urine)
• Helicobacter pylori (drug effects, isolation & purification)
• Humans
• Lipid Peroxidation (physiology)
• Male
• Membrane Proteins
• Middle Aged
• Platelet Activation (physiology)
• Prostaglandin-Endoperoxide Synthases (metabolism)
• Thromboxane B2 (analogs & derivatives, metabolism, urine)