Saucernetin-8 isolated from Saururus chinensis induced the differentiation of human acute promyelocytic leukemia HL-60 cells.

Abstract	The present work was performed to investigate the effects of saucernetin-8 on proliferation and differentiation of human leukemia HL-60 cells as well as the underlying mechanisms for these effects. Saucernetin-8 exhibited a potent antiproliferative activity against HL-60 cells. This compound was also found to be a potent inducer of differentiation in human leukemia derived HL-60 cells through the examination of differentiation markers, as assessed by nitroblue tetrazolium reduction test, esterase activity assay, phagocytic activity assay, morphology change, and expression of CD14 and CD66b surface antigens. These results suggest that saucernetin-8 induces the differentiation of human leukemia cells to granulocytes and monocytes/macrophages lineage. Moreover, DNA flow-cytometry indicated that saucernetin-8 induced a G1 phase arrest of HL-60 cells. The protein and mRNA expression levels of p21 were up-regulated during saucernetin-8-dependent HL-60 cell differentiation, whereas the level of c-myc was down-regulated. Taken together, our results suggest that saucernetin-8 may have potential as a therapeutic agent in human leukemia.
Authors	Bo-Rim Seo, Chae-Bin Yoo, Hee-Juhn Park, Jong-Won Choi, Kyungwon Seo, Seung-Ki Choi, Kyung-Tae Lee
Journal	Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 27 Issue 10 Pg. 1594-8 (Oct 2004) ISSN: 0918-6158 [Print] Japan
PMID	15467202 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antineoplastic Agents CDKN1A protein, human Cell Cycle Proteins Cyclin-Dependent Kinase Inhibitor p21 Furans Lignans Plant Extracts RNA, Messenger saucernetin-8
Topics	Antineoplastic Agents (isolation & purification, pharmacology) Cell Cycle Proteins (biosynthesis, genetics) Cell Differentiation (drug effects) Cell Proliferation (drug effects) Cyclin-Dependent Kinase Inhibitor p21 Down-Regulation Furans (isolation & purification, pharmacology) Genes, myc (drug effects) HL-60 Cells Humans Lignans (isolation & purification, pharmacology) Plant Extracts (pharmacology) RNA, Messenger (biosynthesis) Reverse Transcriptase Polymerase Chain Reaction Saururaceae Up-Regulation

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