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Cephamycin C production is regulated by relA and rsh genes in Streptomyces clavuligerus ATCC27064.

Abstract
The effects of growth rate and nutrient uptake rate on the production of cephamycin C were determined in the parental strain, deltarelA mutant, and deltarsh null mutant of S. clavuligerus. Production of cephamycin C was inversely related to mycelium growth and the phosphate feed rate was more critical for the production of cephamycin C. On the contrary, the production of cephamycin C was completely abolished in the deltarelA mutant, but not in deltarsh mutant. The changes in the cephamycin C production by disruption of the relA and rsh genes are presumably associated with the consequent ability of the mutants to accumulate (p)ppGpp under nutrient starvation. Therefore, it is concluded that the stringent response of S. clavuligerus to starvation for nutrients is governed mainly by RelA rather than Rsh and that the response is more apparently regulated by the limitation of phosphate.
AuthorsWook Jin, Hyo Kyung Kim, Jae Young Kim, Sung Gyun Kang, Sang Hee Lee, Kye Joon Lee
JournalJournal of biotechnology (J Biotechnol) Vol. 114 Issue 1-2 Pg. 81-7 (Oct 19 2004) ISSN: 0168-1656 [Print] Netherlands
PMID15464601 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Cephamycins
  • Phosphates
  • RshA protein, Streptomyces coelicolor
  • Ligases
  • guanosine 3',5'-polyphosphate synthetases
  • Glycerol
  • cephamycin C
Topics
  • Anti-Bacterial Agents (biosynthesis)
  • Bacterial Proteins (genetics, metabolism)
  • Cell Culture Techniques (methods)
  • Cell Proliferation
  • Cephamycins (biosynthesis)
  • Gene Expression Regulation, Bacterial (physiology)
  • Glycerol (metabolism)
  • Ligases (genetics, metabolism)
  • Phosphates (metabolism)
  • Species Specificity
  • Streptomyces (genetics, physiology)

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