Effects of 2-methoxyestradiol on proliferation, apoptosis and PET-tracer uptake in human prostate cancer cell aggregates.

Abstract

The purpose of this study was to investigate the potential use of PET in vivo to record cytotoxic effects of 2-methoxyestradiol (2-ME), an endogenous metabolite of 17beta-estradiol. The anti-proliferative and pro-apoptotic effects of 2-ME on human prostate cancer cell (PC3) aggregates in vitro, were correlated with the uptake of fluoro-deoxy-D-glucose, FMAU and choline labelled with 18F, 11C, or 3H. 2-ME clearly reduced growth of PC3 aggregates and induced apoptosis in a dose-dependent manner. However, the uptake of the putative proliferation markers 11C-FMAU or 3H-choline failed to record the growth inhibitory effects of 2-ME on PC3 cell aggregates. The uptake of 18F-FDG was used as a marker for effects on cellular metabolism and also failed to show any dose-dependent effects in PC3 aggregates. The use of these PET-tracers in vivo is therefore not recommended in order to evaluate the cytotoxic effects of 2-ME on human prostate cancer cells.

• Authors: Padideh Davoodpour, Mats Bergström, Maréne Landström
• Journal: Nuclear medicine and biology (Nucl Med Biol) Vol. 31 Issue 7 Pg. 867-74 (Oct 2004) ISSN: 0969-8051 [Print] United States
• PMID: 15464388 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Radioisotopes
• Radiopharmaceuticals
• Estradiol
• 2-Methoxyestradiol

Topics

• 2-Methoxyestradiol
• Antineoplastic Agents (administration & dosage)
• Apoptosis (drug effects)
• Cell Aggregation (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Dose-Response Relationship, Drug
• Estradiol (administration & dosage, analogs & derivatives)
• Humans
• Male
• Neoplasm Staging (methods)
• Positron-Emission Tomography (methods)
• Prostatic Neoplasms (diagnostic imaging, drug therapy, metabolism, pathology)
• Radioisotopes (pharmacokinetics)
• Radiopharmaceuticals (pharmacokinetics)
• Reproducibility of Results
• Sensitivity and Specificity